Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NRL37_RS25910 Genome accession   NZ_CP102328
Coordinates   5651053..5653143 (-) Length   696 a.a.
NCBI ID   WP_268193449.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain BC12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5646053..5658143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL37_RS25895 (NRL37_25900) - 5647695..5649362 (-) 1668 WP_268193447.1 SPOR domain-containing protein -
  NRL37_RS25900 (NRL37_25905) aroB 5649373..5650476 (-) 1104 WP_268193448.1 3-dehydroquinate synthase -
  NRL37_RS25905 (NRL37_25910) aroK 5650529..5651047 (-) 519 WP_074973697.1 shikimate kinase AroK -
  NRL37_RS25910 (NRL37_25915) pilQ 5651053..5653143 (-) 2091 WP_268193449.1 type IV pilus secretin PilQ Machinery gene
  NRL37_RS25915 (NRL37_25920) pilP 5653198..5653728 (-) 531 WP_152644482.1 type 4a pilus biogenesis lipoprotein PilP -
  NRL37_RS25920 (NRL37_25925) pilO 5653728..5654351 (-) 624 WP_182851206.1 type 4a pilus biogenesis protein PilO -
  NRL37_RS25925 (NRL37_25930) pilN 5654348..5654920 (-) 573 WP_044406731.1 type 4a pilus biogenesis protein PilN -
  NRL37_RS25930 (NRL37_25935) pilM 5654920..5655984 (-) 1065 WP_044406728.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74886.17 Da        Isoelectric Point: 5.1117

>NTDB_id=716209 NRL37_RS25910 WP_268193449.1 5651053..5653143(-) (pilQ) [Metapseudomonas otitidis strain BC12]
MNSSLTRLGLSLLAAMFSPMLLAADLKALDVAALPGGKVELKLGFDGPVVQPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTLVEAKDRTRMIINLSTLVPYSTRVEGNNLYVLVGDAAGAAQQAARTSAPVAAPSQPAAPVKAFAPVGKAI
SNIDFQRGEQGEGNVVITLSDPSVSPNIQEQGGKIRLDFSKTQIPESLRVRLDVKDFATPVQFVSASGSSGDASIVIEPT
GFYDYLAYQTENKLTVSIKPLTQEDMEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQ
NVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQISELAPLRRELIQVNYAKAADIAKLFQSVTSGE
SSSTDERGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVKWRGARVGDNNFVVGG
GAQATGANRIGATTPSGALGTFVDMGATNATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGS
EIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRVLMEVKVTKDAPDFSRQVNGVPPINKNEVNAKVLVNDGETIVIGG
VFSNTQTKSTDKVPFLGDLPYLGRMFKRDVLQDNKAELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=716209 NRL37_RS25910 WP_268193449.1 5651053..5653143(-) (pilQ) [Metapseudomonas otitidis strain BC12]
ATGAACAGCTCCCTAACGCGCCTTGGACTTTCTCTTCTGGCGGCCATGTTTTCACCCATGTTGCTGGCTGCTGACCTCAA
GGCTCTGGACGTCGCGGCCCTGCCGGGCGGAAAGGTCGAGCTGAAACTCGGTTTCGACGGCCCCGTTGTACAGCCGAGAG
GCTATACGATTGAGCAGCCTGCAAGGATCGCATTGGACCTGCCTGGCGTTGCCAACAAACTTGGCAGCAAGAACCGCGAG
CTCGGGGTAGGTAACGCTCGGAGTGTCACTCTTGTTGAGGCGAAAGATCGCACTCGCATGATCATCAACCTGTCGACGTT
GGTGCCTTACAGCACGCGAGTCGAGGGTAACAACCTTTACGTTCTGGTTGGCGATGCTGCCGGTGCTGCTCAACAGGCTG
CACGCACCAGTGCGCCGGTAGCGGCTCCGTCACAGCCTGCTGCACCTGTCAAAGCGTTCGCGCCTGTGGGCAAGGCAATC
AGCAACATTGATTTCCAGAGAGGCGAGCAGGGCGAAGGTAACGTCGTCATTACCCTATCCGACCCCAGTGTGAGTCCCAA
CATCCAAGAGCAGGGTGGAAAGATCCGCCTGGACTTTTCCAAGACCCAAATTCCGGAGTCTCTGCGTGTTCGTCTTGATG
TAAAGGATTTCGCGACGCCGGTTCAGTTCGTCAGTGCCTCTGGCAGCTCGGGTGATGCCAGTATCGTGATCGAGCCGACG
GGCTTCTACGACTACCTGGCCTACCAGACTGAGAACAAGCTGACTGTCAGCATCAAACCGCTGACCCAAGAAGATATGGA
GAAGCGCAAGACCGAGCGCTTTGCCTATTCTGGAGAAAAGCTCTCGCTCAATTTCCAGGATATTGATGTCCGCTCGGTAC
TGCAGCTGATCGCTGACTTCACCGATCTGAACCTGGTTGCGTCAGATACCGTACAAGGCAACATCACGCTCCGCCTGCAG
AACGTACCTTGGGATCAGGCACTTGACCTCGTCCTGAAAACCAAGGGCCTGGACAAGCGCAAGGTCGGTAACGTGCTGCT
GGTTGCACCGGCGGACGAAATCGCTGCCCGTGAACGCCAAGAGCTGGAGTCTCAGAAGCAGATTTCCGAATTGGCTCCGC
TGCGCCGCGAGCTTATCCAGGTCAACTACGCCAAGGCAGCTGATATCGCTAAGCTCTTCCAATCTGTGACTAGCGGTGAG
TCTTCTTCTACGGATGAGCGTGGGTCCATAACGGTCGATGATCGGACGAACAGCATCATCGCTTATCAAACCCAAGATAA
GCTCGACGAACTTCGTCGTATTGTTGCTCAGCTCGATATTCCGGTCCGTCAGGTGATGATTGAAGCGCGAATAGTTGAAG
CTAACGTTGATTACGATAAGTCGCTCGGAGTCAAATGGCGTGGGGCGCGAGTCGGTGATAATAACTTTGTAGTGGGTGGT
GGCGCTCAGGCGACCGGTGCTAATCGCATTGGTGCTACTACCCCGAGCGGGGCTCTCGGCACTTTCGTAGATATGGGGGC
AACCAACGCAACCTCATCTATCGGTATCGGTTTCATCACTGATAACACCATTCTGGATCTGGAGTTGTCCGCTATGGAAA
AAACGGGCAACGGTGAGATCGTGTCTCAGCCGAAGGTCGTGACCTCCGATAAGGAGACGGCAAAAATTCTGAAAGGCTCC
GAGATTCCCTATCAGGAAGCCAGCTCGAGTGGTGCAACGTCTGTTTCATTTAAGGAGGCTGCGCTGTCCTTGGAAGTTAC
ACCTCAGATTACTCCAGATAATAGGGTCTTGATGGAGGTGAAGGTTACTAAGGATGCTCCTGACTTCTCTCGCCAGGTTA
ATGGCGTTCCGCCAATCAACAAGAATGAAGTCAACGCAAAGGTCTTGGTAAACGACGGCGAAACCATTGTGATTGGTGGC
GTATTCTCGAATACTCAGACCAAATCTACTGACAAGGTCCCATTCCTTGGTGATTTGCCCTACCTCGGCCGTATGTTCAA
AAGAGATGTTCTTCAGGACAACAAAGCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAACAACCAGGCTATCGCTG
TGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(304-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.838

100

0.78
  comQ Acinetobacter baylyi ADP1

43.271

99.282

0.43
  pilQ Acinetobacter baumannii strain A118

41.292

100

0.422
  pilQ Acinetobacter baumannii D1279779

41.292

100

0.422
  pilQ Legionella pneumophila strain ERS1305867

38.791

97.414

0.378
  pilQ Legionella pneumophila strain Lp02

38.791

97.414

0.378