Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NRL37_RS25930 Genome accession   NZ_CP102328
Coordinates   5654920..5655984 (-) Length   354 a.a.
NCBI ID   WP_044406728.1    Uniprot ID   A0A0D6SQX4
Organism   Metapseudomonas otitidis strain BC12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5649920..5660984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL37_RS25905 (NRL37_25910) aroK 5650529..5651047 (-) 519 WP_074973697.1 shikimate kinase AroK -
  NRL37_RS25910 (NRL37_25915) pilQ 5651053..5653143 (-) 2091 WP_268193449.1 type IV pilus secretin PilQ Machinery gene
  NRL37_RS25915 (NRL37_25920) pilP 5653198..5653728 (-) 531 WP_152644482.1 type 4a pilus biogenesis lipoprotein PilP -
  NRL37_RS25920 (NRL37_25925) pilO 5653728..5654351 (-) 624 WP_182851206.1 type 4a pilus biogenesis protein PilO -
  NRL37_RS25925 (NRL37_25930) pilN 5654348..5654920 (-) 573 WP_044406731.1 type 4a pilus biogenesis protein PilN -
  NRL37_RS25930 (NRL37_25935) pilM 5654920..5655984 (-) 1065 WP_044406728.1 pilus assembly protein PilM Machinery gene
  NRL37_RS25935 (NRL37_25940) - 5656358..5658589 (+) 2232 WP_394352191.1 penicillin-binding protein 1A -
  NRL37_RS25940 (NRL37_25945) - 5658695..5659963 (-) 1269 WP_044406723.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37701.00 Da        Isoelectric Point: 4.3980

>NTDB_id=716210 NRL37_RS25930 WP_044406728.1 5654920..5655984(-) (pilM) [Metapseudomonas otitidis strain BC12]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNPERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=716210 NRL37_RS25930 WP_044406728.1 5654920..5655984(-) (pilM) [Metapseudomonas otitidis strain BC12]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAACGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGCGTCGGCCAGGCACTGTCGCGTGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGCAGCCGTT
GCTGTGGCGGGTTCAGCCGTCATTACCAAGACGATCGAGATGGATGCAGGGCTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGACCAGTACATTCCTTATCCCCTTGAAGAAGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAC
GAAACCCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTCGAAGTACGCGAAGCAGCTCTGGCGCTT
GCCGGGCTCACCGCGAAAGTCGTGGACGTCGAGGCCTATGCACTTGAGCGCGCTTATGGGCTCCTTGCTTCCCAATTGGG
GAATGGCTCGGAGGATCTGACGGTTGCGATCGTGGATATCGGCGCGACGATGACCACCCTGAGCGTCTTGAACAATGGTC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTTTCTGTC
GAGGAAGCGGGCTTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
GGTTGTCCAGCAAGTTTCGCGGTCGTTGCAGTTCTTTTTCGCAGCGGGCCAGTACAACGATGTCGATTACATCCTCCTGG
CCGGCGGTACTGCCTCTATCCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCACTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6SQX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48