Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRL37_RS05620 Genome accession   NZ_CP102328
Coordinates   1189131..1190834 (-) Length   567 a.a.
NCBI ID   WP_268194240.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain BC12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1184131..1195834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL37_RS05580 (NRL37_05580) - 1184313..1184738 (+) 426 WP_268194233.1 GNAT family N-acetyltransferase -
  NRL37_RS05585 (NRL37_05585) - 1184735..1185211 (+) 477 WP_268194234.1 FAD/FMN-containing dehydrogenase -
  NRL37_RS05590 (NRL37_05590) - 1185208..1185894 (+) 687 WP_268194235.1 energy-coupling factor ABC transporter permease -
  NRL37_RS05595 (NRL37_05595) - 1186005..1186220 (+) 216 WP_268194236.1 hypothetical protein -
  NRL37_RS05600 (NRL37_05600) yacG 1186233..1186430 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  NRL37_RS05605 (NRL37_05605) coaE 1186427..1187038 (-) 612 WP_268194237.1 dephospho-CoA kinase -
  NRL37_RS05610 (NRL37_05610) pilD 1187035..1187907 (-) 873 WP_268194238.1 prepilin peptidase Machinery gene
  NRL37_RS05615 (NRL37_05615) pilC 1187910..1189127 (-) 1218 WP_268194239.1 type II secretion system F family protein Machinery gene
  NRL37_RS05620 (NRL37_05620) pilB 1189131..1190834 (-) 1704 WP_268194240.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NRL37_RS05625 (NRL37_05625) - 1191057..1191554 (+) 498 WP_268194241.1 pilin -
  NRL37_RS05630 (NRL37_05630) - 1191664..1193346 (+) 1683 WP_268194242.1 PglL family O-oligosaccharyltransferase -
  NRL37_RS05640 (NRL37_05640) nadC 1193819..1194667 (-) 849 WP_268194243.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62500.58 Da        Isoelectric Point: 5.4827

>NTDB_id=716195 NRL37_RS05620 WP_268194240.1 1189131..1190834(-) (pilB) [Metapseudomonas otitidis strain BC12]
MSELPTLSGLARQLVQANLLDERTAQSAFIQAQRNKISLVTYLAQNKLLKSRDLAEMAAEQFGIAFMDLASIDKDSQPKD
LVAEKLLRQHRALPLWRRGNKLFIAVSDPTNHQATTDIQFSTGLTTEAILVEDDKLGTALDKFFEGEASGMEDLADVDLD
GLDVEAVEDDKPKSDSGTDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKSKSIDFRVNSLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPPFNIATSVNLIIAQRLARKLCNNCKKEVDVPRDTLLKEGFP
EDKIGTFKIFAPTGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=716195 NRL37_RS05620 WP_268194240.1 1189131..1190834(-) (pilB) [Metapseudomonas otitidis strain BC12]
ATGAGCGAATTGCCCACACTTTCAGGCTTGGCTCGGCAGCTGGTACAGGCCAACCTACTGGATGAAAGAACCGCGCAAAG
CGCGTTTATTCAGGCCCAGCGCAACAAAATTTCCCTTGTGACTTACCTTGCTCAGAACAAGCTTCTGAAAAGCCGCGACC
TGGCCGAAATGGCCGCGGAACAATTCGGCATTGCCTTCATGGACTTGGCAAGCATCGACAAGGACTCACAACCAAAAGAT
CTGGTTGCAGAGAAGCTCCTACGCCAGCACCGTGCATTGCCGCTCTGGAGACGAGGCAACAAGCTATTCATTGCGGTCTC
TGACCCGACCAACCATCAAGCCACGACCGACATTCAGTTCAGCACCGGCTTGACCACCGAAGCGATTCTCGTCGAGGACG
ACAAGCTCGGTACCGCCCTCGATAAGTTTTTTGAAGGCGAAGCCTCCGGCATGGAGGACCTGGCTGATGTCGACCTCGAT
GGGCTGGATGTTGAGGCCGTTGAAGACGACAAACCGAAGTCCGACAGTGGGACGGATGCTGACGACGCTCCCGTTGTACG
CTTCGTCAACAAGATGCTGTTGGATGCCATCAAGGGCGGCTCATCCGACCTCCATTTCGAGCCTTATGAAAAGAGTTACC
GCGTACGACTCCGTACTGACGGCATCTTGCATGAGGTAGCTCGGCCCCCTATACAGCTCGCCAGCCGGATCTCGGCCCGC
CTGAAGGTCATGGCAGCACTCGACATCTCCGAACGCCGCAAGCCACAGGACGGTCGGATCAAGATGAAGATATCCAAGAG
CAAATCCATCGACTTTCGCGTCAACTCGCTACCTACGTTATGGGGCGAGAAAATCGTCATGCGAATTCTGGACCCCTCGA
GTGCACAGATGGGTATTGATGCGCTGGGATATGAAGAGAGCCAGAAGGAGCTCTACCTGAGTGCGCTGAAGCAGCCCCAG
GGAATGATACTGGTAACAGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTACACGGGCCTGAATATTCTCAATACAGT
CGACATCAATATTTCCACTGCGGAAGATCCGGTGGAGATCAACCTGGAAGGGATCAACCAGGTCAACGTCAATCCAAAGC
AAGGCATGGACTTTGCTCAAGCTCTGCGGGCTTTCCTGCGCCAAGACCCTGACGTGATCATGGTGGGTGAGATTCGCGAC
CTGGAAACTGCAGAGATCGCGATCAAAGCCGCCCAGACCGGCCACATGGTGATGTCGACCTTGCACACTAACAGTGCCGC
CGAAACCCTTACCCGATTACGCAACATGGGGGTGCCGCCGTTCAACATCGCAACCTCCGTCAACCTGATCATCGCCCAGC
GTCTTGCCCGCAAGCTCTGCAACAACTGCAAGAAAGAGGTTGATGTCCCCCGTGACACGCTGCTCAAGGAAGGATTCCCC
GAAGACAAAATCGGTACCTTCAAAATTTTCGCCCCGACCGGCTGTGAGAACTGCAAAGGCGGCTACAAGGGCAGGGTCGG
TATTTATGAAGTAGTTAAAAACACGCCCTCGCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCCGCGC
AGATGCGCAAAGATGGTTTCAACGATCTGCGCACCTCGGGGTTGCTGAAGGCGATGCAAGGCGTCACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.777

99.824

0.607
  pilB Acinetobacter baylyi ADP1

59.578

100

0.598
  pilB Legionella pneumophila strain ERS1305867

55.398

99.647

0.552
  pilB Vibrio parahaemolyticus RIMD 2210633

51.323

100

0.513
  pilB Vibrio campbellii strain DS40M4

49.825

100

0.501
  pilB Vibrio cholerae strain A1552

50.534

99.118

0.501
  pilF Neisseria gonorrhoeae MS11

49.201

99.295

0.489
  pilF Thermus thermophilus HB27

37.833

99.295

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

93.298

0.372