Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J71_RS03980 Genome accession   NZ_AP018783
Coordinates   782669..783118 (+) Length   149 a.a.
NCBI ID   WP_005666683.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-3 isolate 15H03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 777669..788118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J71_RS03960 (CHBNV3_07610) rsmE 778133..778870 (-) 738 WP_005666674.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J71_RS03965 (CHBNV3_07620) lnt 778923..780449 (-) 1527 WP_020909963.1 apolipoprotein N-acyltransferase -
  K6J71_RS03970 (CHBNV3_07630) corC 780472..781371 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J71_RS03975 (CHBNV3_07640) ampD 782003..782554 (-) 552 WP_005666680.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J71_RS03980 (CHBNV3_07650) pilA 782669..783118 (+) 450 WP_005666683.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J71_RS03985 (CHBNV3_07660) pilB 783115..784509 (+) 1395 WP_005666685.1 GspE/PulE family protein Machinery gene
  K6J71_RS03990 (CHBNV3_07670) pilC 784506..785726 (+) 1221 WP_005666687.1 type II secretion system F family protein Machinery gene
  K6J71_RS03995 (CHBNV3_07680) pilD 785723..786415 (+) 693 WP_005666688.1 A24 family peptidase Machinery gene
  K6J71_RS04000 (CHBNV3_07690) rho 786470..787732 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15556.89 Da        Isoelectric Point: 8.0922

>NTDB_id=71394 K6J71_RS03980 WP_005666683.1 782669..783118(+) (pilA) [Haemophilus influenzae strain CHBN-V-3 isolate 15H03]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTEASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=71394 K6J71_RS03980 WP_005666683.1 782669..783118(+) (pilA) [Haemophilus influenzae strain CHBN-V-3 isolate 15H03]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGTACAAATGAAACAACAAATTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAGCCTCAGTAAAAACGCAATCAGGTGGCATTACCGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGAGGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

95.973

100

0.96
  pilA Haemophilus influenzae Rd KW20

93.96

100

0.94
  pilA Glaesserella parasuis strain SC1401

53.691

100

0.537
  pilA2 Legionella pneumophila strain ERS1305867

40.136

98.658

0.396
  pilA Vibrio campbellii strain DS40M4

41.135

94.631

0.389
  pilA2 Legionella pneumophila str. Paris

40.288

93.289

0.376

Multiple sequence alignment