Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   POF57_RS04635 Genome accession   NZ_CP116714
Coordinates   855233..855886 (+) Length   217 a.a.
NCBI ID   WP_000461745.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 Cas9 AEKO     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 850233..860886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF57_RS04610 (POF57_04610) - 850399..851229 (+) 831 WP_000735504.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  POF57_RS04615 (POF57_04615) - 851464..853050 (+) 1587 WP_000673089.1 DEAD/DEAH box helicase -
  POF57_RS04620 (POF57_04620) - 853235..853501 (-) 267 WP_000598736.1 GIY-YIG nuclease family protein -
  POF57_RS04625 (POF57_04625) - 853494..854258 (-) 765 WP_000567423.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  POF57_RS04630 (POF57_04630) - 854393..855133 (+) 741 WP_000500220.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  POF57_RS04635 (POF57_04635) comEA/celA/cilE 855233..855886 (+) 654 WP_000461745.1 helix-hairpin-helix domain-containing protein Machinery gene
  POF57_RS04640 (POF57_04640) comEC/celB 855870..858107 (+) 2238 WP_000939900.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  POF57_RS04645 (POF57_04645) - 858233..859042 (+) 810 WP_000153219.1 Cof-type HAD-IIB family hydrolase -
  POF57_RS04650 (POF57_04650) - 859053..859997 (+) 945 WP_000200828.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 23842.23 Da        Isoelectric Point: 9.5543

>NTDB_id=711894 POF57_RS04635 WP_000461745.1 855233..855886(+) (comEA/celA/cilE) [Streptococcus agalactiae strain A909 Cas9 AEKO]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVWAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide


Download         Length: 654 bp        

>NTDB_id=711894 POF57_RS04635 WP_000461745.1 855233..855886(+) (comEA/celA/cilE) [Streptococcus agalactiae strain A909 Cas9 AEKO]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACAATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCTACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTTGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(75-128)

Helix-hairpin-helix motif (HHH_3)

(153-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

44.545

100

0.452
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.243

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae D39

43.243

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae R6

43.243

100

0.442
  comEA/celA/cilE Streptococcus mitis NCTC 12261

42.793

100

0.438
  comEA/celA/cilE Streptococcus mitis SK321

43.182

100

0.438
  comEA Lactococcus lactis subsp. cremoris KW2

38.565

100

0.396
  comEA Streptococcus thermophilus LMD-9

53.896

70.968

0.382