Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NPN27_RS04880 Genome accession   NZ_CP101739
Coordinates   1014779..1016482 (+) Length   567 a.a.
NCBI ID   WP_256518819.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain J15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009779..1021482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPN27_RS04855 (NPN27_04855) cysD 1010152..1011069 (+) 918 WP_125872859.1 sulfate adenylyltransferase subunit CysD -
  NPN27_RS04860 (NPN27_04860) cysN 1011081..1012979 (+) 1899 WP_256518817.1 sulfate adenylyltransferase subunit CysN -
  NPN27_RS04865 (NPN27_04865) - 1013257..1013786 (-) 530 Protein_961 REP-associated tyrosine transposase -
  NPN27_RS22065 - 1014002..1014469 (-) 468 WP_272893401.1 pilin -
  NPN27_RS04880 (NPN27_04880) pilB 1014779..1016482 (+) 1704 WP_256518819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NPN27_RS04885 (NPN27_04885) pilC 1016484..1017701 (+) 1218 WP_256518820.1 type II secretion system F family protein Machinery gene
  NPN27_RS04890 (NPN27_04890) pilD 1017706..1018572 (+) 867 WP_256518821.1 prepilin peptidase Machinery gene
  NPN27_RS04895 (NPN27_04895) coaE 1018704..1019312 (+) 609 WP_080977605.1 dephospho-CoA kinase -
  NPN27_RS04900 (NPN27_04900) yacG 1019309..1019506 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  NPN27_RS04905 (NPN27_04905) - 1019519..1020199 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  NPN27_RS04910 (NPN27_04910) - 1020256..1021296 (-) 1041 WP_256518823.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61899.76 Da        Isoelectric Point: 5.1499

>NTDB_id=711867 NPN27_RS04880 WP_256518819.1 1014779..1016482(+) (pilB) [Stutzerimonas stutzeri strain J15]
MSENTSLSGLARQMVAAGVLDERTAQQAQAQAIRGKTPFVTYVVQNRLAKGRVIAELASEQFGVALLDLAALDKELLPKD
LVSEKLAKQHRVLPLSRRGTKLYIAVSDPANHQSIADIQFSTGLSIEPVLVEDDKLGEAIDKLYDSGATGLEDLGEVDLD
GVDVESVSDDGRTDDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMKLSKNKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRQDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=711867 NPN27_RS04880 WP_256518819.1 1014779..1016482(+) (pilB) [Stutzerimonas stutzeri strain J15]
ATGAGCGAGAATACTTCCCTTTCCGGACTGGCGCGGCAGATGGTGGCAGCTGGTGTGCTGGACGAGAGGACGGCGCAGCA
GGCCCAGGCTCAGGCCATTCGCGGCAAGACACCCTTTGTCACCTACGTCGTCCAGAACCGGTTGGCCAAGGGTCGTGTCA
TTGCCGAACTCGCCTCCGAGCAGTTCGGTGTTGCGCTGCTGGATCTTGCTGCGCTGGACAAGGAGTTGCTGCCCAAGGAC
CTGGTGAGCGAGAAGCTAGCCAAGCAGCACCGGGTATTACCGCTCTCCAGGCGCGGCACTAAGCTGTATATCGCAGTTTC
CGACCCTGCCAACCATCAGTCGATTGCCGATATCCAGTTCAGCACCGGGCTTTCGATCGAGCCAGTACTGGTAGAAGACG
ACAAGCTCGGTGAGGCAATCGACAAGCTTTACGACAGTGGGGCTACTGGGCTGGAGGATCTGGGTGAGGTCGACTTAGAT
GGCGTAGACGTCGAGTCGGTTAGTGATGACGGTCGGACGGATGATGCAGGGGAGGCCGCGGACGATGCGCCCGTTGTGCG
CTTCGTCAACAAGATGTTGCTCGATGCCATTCGTGGAGGCTCATCTGATCTCCACTTTGAGCCTTATGAGAAAGCCTACC
GGGTACGGTTACGTACTGATGGCATTTTGCATGAGGTGGCGCGTCCTCCAATACAGTTGGCGTCCAGGATTTCTGCGCGC
CTCAAGGTAATGGCAGGGCTGGATATTTCCGAGCGTCGCAAACCGCAGGATGGCCGCATCAAGATGAAGCTGTCCAAAAA
CAAGGCAATTGATTTTCGCGTCAATACGCTACCCACTCTGTGGGGCGAGAAGATCGTAATGCGAATCCTTGATCCGTCCA
GCGCACAGATGGGCATCGATGCGTTGGGATATGAAGAGGACCAGAAAGAGCTTTATATGAATGCCCTGAAGCAACCGCAG
GGCATGATTTTGGTTACCGGCCCGACCGGCTCTGGTAAGACGGTGTCGCTCTATACCGGGCTGAACATCCTCAATACGGT
CGACGTGAATATCTCGACCGCAGAAGATCCTGTAGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCACGTC
AGGGGATGGACTTTTCCCAGGCGCTACGCGCGTTTCTGCGTCAGGATCCAGACATCATCATGGTGGGTGAGATCCGTGAT
CTGGAGACCGCTGAGATTGCCATCAAGGCGGCGCAGACGGGCCACATGGTCATGTCGACGCTACACACCAACAGCGCCGC
GGAAACTCTGACCCGTCTTCGCAACATGGGGGTGGCCGCGTTCAATATTGCGACCTCGGTCAACCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGCTGCAAGAAGGAGATCGATCTGCCACGCGAGGCGCTGTTGGAAGAGGGTTTCCCT
GCCGACAAGATTGGGACGTTCAAAATCTATGGCCCGGTCGGCTGCGACAATTGCAAGGGCGGTTACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCGTTGGCCAGGATTATCATGGAGGACGGCAATTCCATCGACATTTCCACCC
AGATGCGACAAGACGGCTTTAACGATCTGCGTACTTCGGCTCTAGTTAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.965

100

0.6
  pilB Acinetobacter baylyi ADP1

59.325

99.295

0.589
  pilB Legionella pneumophila strain ERS1305867

55.245

100

0.557
  pilB Vibrio campbellii strain DS40M4

48.09

100

0.489
  pilB Vibrio cholerae strain A1552

53.411

90.476

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

52.399

91.887

0.481
  pilF Neisseria gonorrhoeae MS11

48.49

99.295

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.798

96.12

0.363