Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NPN27_RS03060 Genome accession   NZ_CP101739
Coordinates   643542..645662 (+) Length   706 a.a.
NCBI ID   WP_017244293.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain J15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638542..650662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPN27_RS03040 (NPN27_03040) comM 640705..641769 (+) 1065 WP_048328486.1 pilus assembly protein PilM Machinery gene
  NPN27_RS03045 (NPN27_03045) pilN 641769..642341 (+) 573 WP_014595705.1 type 4a pilus biogenesis protein PilN -
  NPN27_RS03050 (NPN27_03050) pilO 642338..642964 (+) 627 WP_017244292.1 type 4a pilus biogenesis protein PilO -
  NPN27_RS03055 (NPN27_03055) pilP 642961..643488 (+) 528 WP_013981653.1 type 4a pilus biogenesis lipoprotein PilP -
  NPN27_RS03060 (NPN27_03060) pilQ 643542..645662 (+) 2121 WP_017244293.1 type IV pilus secretin PilQ Machinery gene
  NPN27_RS03065 (NPN27_03065) aroK 645666..646211 (+) 546 WP_017244294.1 shikimate kinase AroK -
  NPN27_RS03070 (NPN27_03070) aroB 646235..647338 (+) 1104 WP_017244295.1 3-dehydroquinate synthase -
  NPN27_RS03075 (NPN27_03075) - 647349..648944 (+) 1596 WP_256518620.1 AAA family ATPase -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 75704.90 Da        Isoelectric Point: 4.7044

>NTDB_id=711863 NPN27_RS03060 WP_017244293.1 643542..645662(+) (pilQ) [Stutzerimonas stutzeri strain J15]
MNTTLSRLGVSLLAAFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESSGAANPVSASAAITAPLVKPAPVKPFAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNKLTISVKPLTEEEADKRKTDRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTSNGAV
AEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKALGVRWGGSYTNDNWGLYGKDGA
IGRDEDGGRYLLPGTDKVGNFTLEDALAPVPFVDLGVLNSTAGIGIGYVSDHGILDLQLSAMEKTGNGEVVSQPKVVTAD
KETAKILKGAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLV
TDGETIVIGGVFSNSQISSIEKVPFLGDVPFVGRLFRRDIVEDKKSELLVFLTPRILNYRAVAVGQ

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=711863 NPN27_RS03060 WP_017244293.1 643542..645662(+) (pilQ) [Stutzerimonas stutzeri strain J15]
ATGAACACTACCCTTTCTCGCCTGGGCGTATCTCTGCTGGCGGCATTTATTTCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCCTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTCGCGTCACCTCGTG
GCTACACGCTGGATCAGCCCGCGCGCATTGCCCTGGATCTTCCAGGCGTATCCAACAACCTGGGGGCCAAAAATCGGGAG
CTGGGCGTTGGTAATGCACGCAGCGTAACGGTCGTCGAGGCTCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGACGGGAACAATCTGTTTGTCGTGCTGGGAGAAAGTTCGGGAGCGGCCAACCCTGTGT
CTGCATCCGCCGCGATCACTGCTCCGCTTGTCAAGCCAGCCCCGGTAAAGCCGTTTGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAGGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGTGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCACTGCGTGTTCGGCTCGACGTCCAGG
ATTTTGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGCGGCGCCAGTATCGCCATCGAGCCTACAGGGCGA
TACGATTATCTCGCCTACCAGACAGACAACAAGCTGACCATCAGCGTAAAGCCCCTTACAGAAGAAGAGGCCGATAAGCG
CAAGACAGACCGTTTTGCCTATTCCGGTGAAAAACTGTCGCTGAACTTCCAGGATATTGATGTTCGTTCCGTACTCCAGT
TGATCGCTGATTTTACTGACCTGAACTTGGTTGCCAGCGACACCGTCAGTGGCAACATCACCCTTCGTTTGCAGAACGTG
CCTTGGGACCAAGCGCTCGATCTGGTTTTGAAAACCAAGGGGCTGGATAAGCGGCAAGTCGGGAACGTGCTGCTCGTTGC
ACCTGCTGACGAAATCGCTGCGCGTGAGCGCCAAGAGCTCGAGTCGCAGCGCCAGATTGCCGAGCTTGCACCGTTGCGTC
GTGAGGTCGTGCAAGTCAACTATGCCAAGGCTGCGGACATTGCTCGCCTGTTCCAGTCAGTGACGAGCAACGGCGCCGTT
GCAGAGGATCGTGGCTCTATAGCGGTGGATGATCGCACCAATAACATCATCGCTTATCAAACGCAGGAGCGACTTGACGA
GCTGCGTCGCATCGTCGCTCAGCTCGACATTCCTGTGCGGCAAGTGATGATCGAGGCGCGCATTGTTGAGGCGAATGTGG
GCTACGACAAAGCGCTTGGGGTTCGTTGGGGGGGTAGCTACACCAACGACAATTGGGGCTTGTATGGCAAGGACGGTGCC
ATCGGTCGTGATGAAGATGGCGGACGATATCTTCTTCCAGGTACAGACAAAGTCGGCAATTTTACCCTTGAAGATGCGTT
GGCCCCGGTTCCATTTGTCGATTTAGGAGTGCTTAACAGCACAGCCGGTATTGGGATTGGCTATGTCTCGGACCATGGAA
TCTTAGACCTTCAATTGTCGGCGATGGAGAAAACCGGTAACGGTGAAGTCGTCTCCCAACCAAAGGTGGTTACTGCGGAT
AAAGAAACGGCCAAGATCTTGAAAGGCGCGGAAATCCCCTACCAAGAAGCCAGTTCGAGTGGCGCAACGTCGACATCCTT
CAAGGAGGCAGCTCTTTCGCTTGAGGTGACGCCGCAGATCACGCCGGACAACCGCATTATCATGGAGGTCAAGGTCACCA
AGGATGCCCCTGATTTCGCTAACGCTCTGAATGGGGTGCCGCCGATCAACAAGAACGAAGTCAACGCCAAAGTTCTTGTT
ACCGATGGAGAAACAATCGTCATCGGTGGTGTATTCTCCAACAGCCAGATCAGCAGCATTGAAAAAGTGCCGTTCCTGGG
TGACGTTCCGTTCGTTGGTCGCCTCTTCCGACGGGACATCGTGGAAGATAAAAAATCCGAGCTTCTGGTCTTCTTGACCC
CTAGAATCCTCAACTACCGAGCGGTCGCCGTGGGCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

AMIN domain (AMIN)

(38-123)

Bacterial type II and III secretion system protein (Secretin)

(541-697)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.131

100

0.755
  comQ Acinetobacter baylyi ADP1

42.098

98.584

0.415
  pilQ Acinetobacter baumannii strain A118

40.533

100

0.409
  pilQ Acinetobacter baumannii D1279779

40.533

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.088

96.317

0.367
  pilQ Legionella pneumophila strain Lp02

37.941

96.317

0.365