Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NFC81_RS04175 Genome accession   NZ_CP101717
Coordinates   951885..953588 (-) Length   567 a.a.
NCBI ID   WP_304996280.1    Uniprot ID   A0AB38YIG3
Organism   Salinispirillum sp. LH 10-3-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 946885..958588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFC81_RS04145 (NFC81_04145) argJ 947235..948449 (+) 1215 WP_304996273.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  NFC81_RS04150 (NFC81_04150) mutT 948412..948888 (+) 477 WP_304996274.1 8-oxo-dGTP diphosphatase MutT -
  NFC81_RS04155 (NFC81_04155) yacG 948903..949112 (-) 210 WP_304996275.1 DNA gyrase inhibitor YacG -
  NFC81_RS04160 (NFC81_04160) coaE 949135..949758 (-) 624 WP_304996276.1 dephospho-CoA kinase -
  NFC81_RS04165 (NFC81_04165) pilD 949743..950618 (-) 876 WP_304996278.1 A24 family peptidase Machinery gene
  NFC81_RS04170 (NFC81_04170) pilC 950618..951826 (-) 1209 WP_304996279.1 type II secretion system F family protein Machinery gene
  NFC81_RS04175 (NFC81_04175) pilB 951885..953588 (-) 1704 WP_304996280.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NFC81_RS04180 (NFC81_04185) - 953850..954335 (+) 486 WP_304996281.1 pilin -
  NFC81_RS04185 (NFC81_04190) - 954479..955786 (+) 1308 WP_304996282.1 O-antigen ligase family protein -
  NFC81_RS04190 (NFC81_04195) nadC 955756..956610 (-) 855 WP_304996283.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NFC81_RS04195 (NFC81_04200) - 956651..957292 (+) 642 WP_304996284.1 hypothetical protein -
  NFC81_RS04200 (NFC81_04205) trxB 957435..958397 (+) 963 WP_304996285.1 thioredoxin-disulfide reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61840.13 Da        Isoelectric Point: 5.0693

>NTDB_id=711705 NFC81_RS04175 WP_304996280.1 951885..953588(-) (pilB) [Salinispirillum sp. LH 10-3-1]
MTIALSGMNKRLAEQHIVSADSLMDAQKAAARDKVSIITYLVQNGMAKASDLATIASDELGVPVFDLRAFDMEQLPRDLV
DIKLLQKHRALPLIKRANRLFIGVSDPTALKALEDIRFATGLSTELVIVEDDILGEMLDKAADAGATDLGDFEDGEFEEL
DVVDAEQEDNERNDISGGTDDAPVVRFVQQMLLSAIKKGASDLHFEPYERAYRVRFRVDGVLQTMSKPPIQLGPKIAARL
KILSNMDISERRIPQDGRIKLRISKNKAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEEDQKAIYLNALSKPQG
MILVTGPTGSGKTVSLYTGLNIVNQEGVNISTAEDPVEINLEGINQVNVNAKVGLDFAVALRSFLRQDPDIIMVGEIRDL
ETASIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPSFNLATTINVIIAQRLARRLCSACKKPADVPAEALREQGFSD
ELIKQAKLFQPVGCSACNKGYKGRVGVYEVVRITPRISKIIMEGGNAIELDAAMREDGFASLRQSGLMKAAQGVTSLEEI
NRVTIDN

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=711705 NFC81_RS04175 WP_304996280.1 951885..953588(-) (pilB) [Salinispirillum sp. LH 10-3-1]
ATGACCATAGCCCTGTCCGGAATGAATAAGCGTCTCGCCGAACAGCACATAGTGAGTGCTGACTCCCTTATGGATGCGCA
GAAAGCTGCGGCACGCGACAAGGTGTCGATCATTACTTATTTGGTGCAAAACGGCATGGCCAAGGCCAGTGATCTAGCAA
CCATAGCCTCTGACGAGCTTGGGGTGCCCGTTTTTGATCTGCGTGCCTTTGATATGGAACAGTTGCCCCGCGACCTGGTC
GATATCAAGCTATTGCAGAAGCATCGAGCGTTGCCACTGATTAAGCGTGCAAATCGTTTATTTATTGGCGTGTCAGACCC
AACGGCCCTGAAGGCACTTGAAGATATACGCTTTGCTACAGGCCTGAGTACCGAACTGGTTATTGTTGAAGACGACATTT
TGGGCGAAATGCTCGACAAAGCGGCTGATGCTGGCGCCACCGATCTCGGTGATTTTGAAGATGGTGAATTTGAGGAACTC
GATGTTGTAGACGCCGAGCAAGAAGACAATGAGCGCAACGACATCAGTGGCGGCACCGACGACGCGCCAGTGGTGCGCTT
TGTGCAGCAAATGCTCTTGAGCGCCATCAAAAAAGGCGCATCGGACTTGCATTTTGAACCGTACGAGCGAGCTTACCGCG
TGCGCTTCCGAGTGGATGGCGTGTTGCAGACCATGTCGAAGCCGCCCATACAACTCGGACCCAAGATTGCCGCGCGCCTG
AAGATTTTGTCCAACATGGACATCTCCGAACGCCGCATACCGCAAGACGGTCGTATCAAACTGCGTATTTCCAAAAACAA
GGCCATCGATTTCCGGGTTAACACGTTGCCGACTTTATTTGGCGAGAAGATCGTTCTCCGTATCCTCGACCCTTCCAGTG
CCAAGCTGGGTATTGATGCATTGGGTTACGAAGAAGACCAGAAAGCAATTTACTTAAACGCTCTGAGCAAGCCTCAGGGT
ATGATTCTGGTCACCGGCCCCACAGGTTCTGGTAAAACGGTATCGCTCTACACCGGCTTGAACATCGTCAACCAAGAAGG
CGTCAATATTTCCACGGCGGAAGATCCGGTGGAAATCAACTTGGAAGGCATCAACCAGGTGAACGTGAATGCCAAGGTCG
GGCTCGACTTCGCAGTAGCACTTCGGTCGTTCCTGCGCCAAGACCCCGACATCATCATGGTGGGTGAGATTCGTGACTTA
GAAACGGCGTCAATCGCTATTAAGGCAGCACAAACCGGCCACATGGTGATGTCGACCCTGCACACCAATAGCGCGGCTGA
AACCTTGACCCGCTTGCTCAATATGGGCGTGCCCTCGTTCAACTTGGCGACCACCATCAATGTCATTATCGCCCAACGTC
TGGCACGTCGCTTGTGTAGCGCCTGTAAAAAACCGGCGGATGTACCGGCGGAAGCACTGCGTGAACAAGGCTTCAGCGAT
GAACTGATCAAACAAGCCAAGTTATTTCAACCCGTGGGTTGTAGTGCGTGTAATAAAGGCTACAAGGGGCGTGTCGGTGT
CTATGAGGTGGTCCGCATCACGCCACGTATTTCCAAAATCATCATGGAAGGCGGCAATGCGATAGAGCTGGATGCCGCCA
TGCGTGAAGACGGCTTCGCGAGCCTGCGGCAGTCGGGATTAATGAAAGCGGCTCAAGGTGTGACCAGCCTCGAAGAAATA
AACCGCGTAACTATTGATAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.947

99.647

0.617
  pilB Acinetobacter baylyi ADP1

59.075

99.118

0.586
  pilB Legionella pneumophila strain ERS1305867

50.53

99.824

0.504
  pilB Vibrio cholerae strain A1552

51.571

95.414

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

50.455

96.825

0.489
  pilB Vibrio campbellii strain DS40M4

50.457

96.473

0.487
  pilF Neisseria gonorrhoeae MS11

50.654

94.356

0.478
  pilF Thermus thermophilus HB27

38.175

94.709

0.362