Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NM620_RS01895 Genome accession   NZ_CP101694
Coordinates   382388..384073 (-) Length   561 a.a.
NCBI ID   WP_256383601.1    Uniprot ID   -
Organism   Photobacterium toruni strain WD2103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 377388..389073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM620_RS01865 mutT 378024..378461 (-) 438 WP_256383597.1 8-oxo-dGTP diphosphatase MutT -
  NM620_RS01870 yacG 378581..378781 (-) 201 WP_080174490.1 DNA gyrase inhibitor YacG -
  NM620_RS01875 zapD 378789..379526 (-) 738 WP_080174491.1 cell division protein ZapD -
  NM620_RS01880 coaE 379573..380178 (-) 606 WP_256383598.1 dephospho-CoA kinase -
  NM620_RS01885 pilD 380182..381054 (-) 873 WP_256383599.1 A24 family peptidase Machinery gene
  NM620_RS01890 pilC 381114..382334 (-) 1221 WP_256383600.1 type II secretion system F family protein Machinery gene
  NM620_RS01895 pilB 382388..384073 (-) 1686 WP_256383601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NM620_RS01900 - 384076..384465 (-) 390 WP_256383602.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NM620_RS01905 - 384961..386337 (-) 1377 WP_256383603.1 MATE family efflux transporter -
  NM620_RS01910 - 386461..387393 (-) 933 WP_256383604.1 carbamate kinase -
  NM620_RS01915 ptcA 387467..388513 (-) 1047 WP_080174499.1 putrescine carbamoyltransferase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62620.13 Da        Isoelectric Point: 5.0375

>NTDB_id=711569 NM620_RS01895 WP_256383601.1 382388..384073(-) (pilB) [Photobacterium toruni strain WD2103]
MQTNLASILFQADLISREQEQQIVESVTAEGINVPTAITRLGIMADDELARSLSHLFNLDRVDVHHYDFQGCCQSVYQRE
LVLRHRVLPLAQDNSTLFVGLSDPTNVDAQDEFRFAIGKNVEPLLLDNKQLESAIRRVYGSDIGEVSNNRQISDEDLSNL
IDINDLELDESTDLSRDSAPVTRYINQVLMDALRRKASDIHFEPYETSYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDTLAIDLRVSTLPTLWGEKVVLRILDSSSASLDIDILGYSETQKQAYLTALQRPQGMIL
MTGPTGSGKTVSLYTGLSILNSDERNISTAEDPVEINLQGINQVQINNQAGLGFAQALRSFLRQDPDVVMVGEIRDLETA
EIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCSHCKQPHLLDDLTCERLKLPLDTT
VYQAHKEGCDDCNKGYIGRVGIYEVMPFSRDIAEALMQKATSLQLEDIAASQGMQRLRESGIDKLVEGITSLTELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=711569 NM620_RS01895 WP_256383601.1 382388..384073(-) (pilB) [Photobacterium toruni strain WD2103]
ATGCAAACCAACCTCGCCTCGATCCTGTTCCAAGCTGACTTAATCAGCCGCGAACAGGAGCAGCAAATAGTCGAGTCGGT
GACGGCGGAAGGGATCAATGTCCCGACCGCCATAACTCGCTTGGGCATTATGGCGGATGATGAACTGGCACGTTCATTAT
CGCACCTGTTCAATCTCGATCGGGTTGATGTCCACCACTATGACTTTCAAGGTTGCTGTCAAAGTGTCTACCAACGTGAA
CTGGTGTTACGCCATCGCGTGCTGCCGCTTGCGCAAGACAATAGCACCCTTTTCGTCGGCCTTAGCGATCCTACCAATGT
CGATGCGCAGGATGAGTTTCGCTTCGCTATTGGTAAGAACGTTGAACCATTATTATTAGATAATAAACAACTTGAATCCG
CGATTCGACGGGTTTACGGCAGTGATATTGGCGAGGTTAGTAATAACCGTCAAATCAGTGATGAAGATCTGAGTAACTTA
ATTGATATTAACGATCTAGAGCTTGATGAATCCACTGATTTAAGCCGAGACAGCGCCCCCGTTACTCGTTATATCAACCA
AGTATTAATGGATGCGCTACGGCGTAAAGCGTCAGATATTCATTTTGAACCTTATGAAACCAGCTATCGAATTCGATTTC
GGTGTGATGGTATTTTACATCAGCATTTAACGCCACCAGCAAGTTTATCGCGGCGGTTATCGACCCGTTTAAAGGTCATG
TCAAAACTCAATATTGCCGAGCGTCGCCAACCGCAAGATGGACGGATAAAACTTAAATTATCTGACACATTGGCAATTGA
TTTACGCGTTTCTACTCTGCCAACCTTGTGGGGCGAAAAAGTCGTACTGCGAATTTTAGACAGTTCCAGTGCCAGCTTAG
ACATTGATATTTTAGGCTATAGCGAAACCCAAAAGCAGGCTTATTTAACCGCCCTCCAGCGCCCGCAAGGCATGATCCTA
ATGACAGGTCCAACCGGGAGTGGTAAGACCGTATCGCTATACACAGGCTTAAGTATTTTAAACTCTGATGAGCGCAATAT
TTCTACCGCTGAAGATCCAGTAGAGATCAACCTGCAAGGCATTAACCAAGTTCAAATTAATAATCAAGCGGGACTTGGAT
TTGCCCAAGCCTTGCGATCGTTTCTGCGTCAAGATCCTGATGTGGTGATGGTCGGTGAAATTCGTGATTTAGAAACCGCT
GAAATCGCGGTAAAGGCAGCGCAAACAGGGCACTTAGTATTATCAACGCTGCACACCAACTCCGCCGCCGAAACGATTAC
CCGTTTAACCAACATGGGAGTGGAAGATTTTAATCTCGCATCATCACTGAGTTTAATTATAGCGCAACGATTAGCGCGCC
GATTATGCAGTCATTGCAAACAACCACATTTGCTTGATGATCTCACCTGTGAGCGTTTAAAACTGCCACTCGACACAACT
GTCTATCAAGCGCATAAAGAAGGCTGTGATGATTGTAATAAAGGCTACATTGGACGAGTAGGTATTTATGAAGTGATGCC
ATTTAGCCGTGACATTGCCGAAGCATTAATGCAAAAAGCCACCAGCTTACAATTAGAAGATATTGCCGCCAGCCAAGGCA
TGCAACGACTGCGCGAATCAGGCATCGATAAATTAGTTGAAGGCATTACTAGCCTGACGGAATTACAACGGGTACTGCAA
CTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.321

99.822

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

61.032

100

0.611
  pilB Vibrio campbellii strain DS40M4

59.643

99.822

0.595
  pilB Acinetobacter baumannii D1279779

53.565

92.513

0.496
  pilB Acinetobacter baylyi ADP1

51.451

92.157

0.474
  pilB Legionella pneumophila strain ERS1305867

46.986

100

0.472
  pilF Neisseria gonorrhoeae MS11

45.115

100

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

40.194

91.8

0.369
  pilF Thermus thermophilus HB27

36.46

100

0.367