Detailed information NTG0711446

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NO220_RS12345
• Genome accession: NZ_CP101661
• Coordinates: 2539357..2539971 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain B408
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2534357..2544971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NO220_RS12315 (NO220_12315)	rpsT	2534657..2534923 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NO220_RS12320 (NO220_12320)	holA	2534940..2535981 (-)	1042	Protein_2379	DNA polymerase III subunit delta	-
NO220_RS12325 (NO220_12325)	-	2536021..2536175 (-)	155	Protein_2380	hypothetical protein	-
NO220_RS12330 (NO220_12330)	-	2536216..2536350 (+)	135	WP_003152870.1	YqzM family protein	-
NO220_RS12335 (NO220_12335)	comEC	2536369..2538720 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NO220_RS12340 (NO220_12340)	-	2538721..2539290 (-)	570	WP_003152868.1	ComE operon protein 2	-
NO220_RS12345 (NO220_12345)	comEA	2539357..2539971 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
NO220_RS12350 (NO220_12350)	comER	2540030..2540851 (+)	822	WP_043867298.1	late competence protein ComER	-
NO220_RS12355 (NO220_12355)	-	2540920..2541657 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
NO220_RS12360 (NO220_12360)	rsfS	2541654..2542010 (-)	357	WP_003152864.1	ribosome silencing factor	-
NO220_RS12365 (NO220_12365)	yqeK	2542007..2542588 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NO220_RS12370 (NO220_12370)	-	2542578..2543147 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
NO220_RS12375 (NO220_12375)	yhbY	2543158..2543448 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NO220_RS12380 (NO220_12380)	aroE	2543442..2544284 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=711446 NO220_RS12345 WP_043867297.1 2539357..2539971(-) (comEA) [Bacillus amyloliquefaciens strain B408]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=711446 NO220_RS12345 WP_043867297.1 2539357..2539971(-) (comEA) [Bacillus amyloliquefaciens strain B408]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363