Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   NO220_RS12335 Genome accession   NZ_CP101661
Coordinates   2536369..2538720 (-) Length   783 a.a.
NCBI ID   WP_043867296.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain B408     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531369..2543720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NO220_RS12300 (NO220_12300) - 2531732..2532070 (-) 339 WP_043867645.1 YqxA family protein -
  NO220_RS12305 (NO220_12305) spoIIP 2532087..2533280 (-) 1194 WP_014305446.1 stage II sporulation protein P -
  NO220_RS12310 (NO220_12310) gpr 2533348..2534454 (-) 1107 WP_003152878.1 GPR endopeptidase -
  NO220_RS12315 (NO220_12315) rpsT 2534657..2534923 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NO220_RS12320 (NO220_12320) holA 2534940..2535981 (-) 1042 Protein_2379 DNA polymerase III subunit delta -
  NO220_RS12325 (NO220_12325) - 2536021..2536175 (-) 155 Protein_2380 hypothetical protein -
  NO220_RS12330 (NO220_12330) - 2536216..2536350 (+) 135 WP_003152870.1 YqzM family protein -
  NO220_RS12335 (NO220_12335) comEC 2536369..2538720 (-) 2352 WP_043867296.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NO220_RS12340 (NO220_12340) - 2538721..2539290 (-) 570 WP_003152868.1 ComE operon protein 2 -
  NO220_RS12345 (NO220_12345) comEA 2539357..2539971 (-) 615 WP_043867297.1 helix-hairpin-helix domain-containing protein Machinery gene
  NO220_RS12350 (NO220_12350) comER 2540030..2540851 (+) 822 WP_043867298.1 late competence protein ComER -
  NO220_RS12355 (NO220_12355) - 2540920..2541657 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  NO220_RS12360 (NO220_12360) rsfS 2541654..2542010 (-) 357 WP_003152864.1 ribosome silencing factor -
  NO220_RS12365 (NO220_12365) yqeK 2542007..2542588 (-) 582 WP_003152863.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NO220_RS12370 (NO220_12370) - 2542578..2543147 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  NO220_RS12375 (NO220_12375) yhbY 2543158..2543448 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86640.62 Da        Isoelectric Point: 9.0078

>NTDB_id=711444 NO220_RS12335 WP_043867296.1 2536369..2538720(-) (comEC) [Bacillus amyloliquefaciens strain B408]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGNLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=711444 NO220_RS12335 WP_043867296.1 2536369..2538720(-) (comEC) [Bacillus amyloliquefaciens strain B408]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTCTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAAATCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATCGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.477

98.595

0.557