Detailed information NTG0711249

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: POF63_RS04280
• Genome accession: NZ_CP116708
• Coordinates: 782881..783534 (+)
• Length: 217 a.a.
• NCBI ID: WP_000461742.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 sCas9
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 777881..788534

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF63_RS04255 (POF63_04255)	-	778048..778878 (+)	831	WP_000735512.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
POF63_RS04260 (POF63_04260)	-	779113..780699 (+)	1587	WP_000673082.1	DEAD/DEAH box helicase	-
POF63_RS04265 (POF63_04265)	-	780884..781150 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
POF63_RS04270 (POF63_04270)	-	781143..781907 (-)	765	WP_000611114.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
POF63_RS04275 (POF63_04275)	-	782042..782782 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
POF63_RS04280 (POF63_04280)	comEA/celA/cilE	782881..783534 (+)	654	WP_000461742.1	helix-hairpin-helix domain-containing protein	Machinery gene
POF63_RS04285 (POF63_04285)	comEC/celB	783518..785755 (+)	2238	WP_000939909.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
POF63_RS04290 (POF63_04290)	-	785881..786690 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
POF63_RS04295 (POF63_04295)	-	786701..787645 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 217 a.a.
• Molecular weight: 23728.08 Da
• Isoelectric Point: 9.2366

>NTDB_id=711249 POF63_RS04280 WP_000461742.1 782881..783534(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLTNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide

• Length: 654 bp

>NTDB_id=711249 POF63_RS04280 WP_000461742.1 782881..783534(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACGATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAACCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCCACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (153-215)

• SLBB domain (SLBB) (75-128)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45.045	100	0.461
comEA/celA/cilE	Streptococcus pneumoniae Rx1	44.144	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae D39	44.144	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae R6	44.144	100	0.452
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.694	100	0.447
comEA/celA/cilE	Streptococcus mitis SK321	43.636	100	0.442
comEA	Lactococcus lactis subsp. cremoris KW2	39.462	100	0.406
comEA	Streptococcus thermophilus LMD-9	55.195	70.968	0.392
comEA	Latilactobacillus sakei subsp. sakei 23K	33.054	100	0.364