Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PIB97_RS03835 Genome accession   NZ_CP116197
Coordinates   801328..802821 (+) Length   497 a.a.
NCBI ID   WP_000249348.1    Uniprot ID   B7LGA7
Organism   Escherichia coli strain DETEC-C31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796328..807821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB97_RS03815 (PIB97_03815) pilD 796976..797785 (+) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene
  PIB97_RS03820 (PIB97_03820) gspS2 797851..798261 (+) 411 WP_021557202.1 type II secretion system pilot lipoprotein GspS-beta -
  PIB97_RS03825 (PIB97_03825) gspC 798279..799238 (+) 960 WP_024227187.1 type II secretion system protein GspC -
  PIB97_RS03830 (PIB97_03830) gspD 799268..801328 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  PIB97_RS03835 (PIB97_03835) pilB/pilB1 801328..802821 (+) 1494 WP_000249348.1 type II secretion system ATPase GspE Machinery gene
  PIB97_RS03840 (PIB97_03840) gspF 802821..804044 (+) 1224 WP_021557200.1 type II secretion system inner membrane protein GspF -
  PIB97_RS03845 (PIB97_03845) gspG 804061..804516 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  PIB97_RS03850 (PIB97_03850) gspH 804520..805083 (+) 564 WP_001613816.1 type II secretion system minor pseudopilin GspH -
  PIB97_RS03855 (PIB97_03855) gspI 805080..805451 (+) 372 WP_021557199.1 type II secretion system minor pseudopilin GspI -
  PIB97_RS03860 (PIB97_03860) gspJ 805448..806053 (+) 606 WP_021557198.1 type II secretion system minor pseudopilin GspJ -
  PIB97_RS03865 (PIB97_03865) gspK 806050..807027 (+) 978 WP_021557197.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.36 Da        Isoelectric Point: 6.0390

>NTDB_id=709138 PIB97_RS03835 WP_000249348.1 801328..802821(+) (pilB/pilB1) [Escherichia coli strain DETEC-C31]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=709138 PIB97_RS03835 WP_000249348.1 801328..802821(+) (pilB/pilB1) [Escherichia coli strain DETEC-C31]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTAATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTAACGGGGCCGACGGGTTCCGGAAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAATATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGAATTGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCGCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCATAAAGGTTTCCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362