Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NK667_RS25130 Genome accession   NZ_CP101125
Coordinates   5725897..5727984 (-) Length   695 a.a.
NCBI ID   WP_054616459.1    Uniprot ID   -
Organism   Pseudomonas nunensis strain In5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5720897..5732984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK667_RS25115 (NK667_25115) - 5722522..5724111 (-) 1590 WP_054616457.1 AAA family ATPase -
  NK667_RS25120 (NK667_25120) aroB 5724122..5725222 (-) 1101 WP_054616458.1 3-dehydroquinate synthase -
  NK667_RS25125 (NK667_25125) aroK 5725374..5725892 (-) 519 WP_054054596.1 shikimate kinase AroK -
  NK667_RS25130 (NK667_25130) pilQ 5725897..5727984 (-) 2088 WP_054616459.1 type IV pilus secretin PilQ Machinery gene
  NK667_RS25135 (NK667_25135) - 5727998..5728525 (-) 528 WP_054054599.1 pilus assembly protein PilP -
  NK667_RS25140 (NK667_25140) pilO 5728522..5729145 (-) 624 WP_054054601.1 type 4a pilus biogenesis protein PilO -
  NK667_RS25145 (NK667_25145) - 5729142..5729708 (-) 567 WP_054616460.1 PilN domain-containing protein -
  NK667_RS25150 (NK667_25150) comM 5729708..5730772 (-) 1065 WP_054054605.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75173.58 Da        Isoelectric Point: 5.8321

>NTDB_id=708905 NK667_RS25130 WP_054616459.1 5725897..5727984(-) (pilQ) [Pseudomonas nunensis strain In5]
MNRIFSTLGISLWIALLSPMVQAATLNALDVAALPGDRVELKLTFDGPPPPPHGYTTESPARIALDLPGVVSKLTNKNRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYSSRVEGNNLFVVVGQGAKGTTAKPVASAPRPATAKPAPAKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILGFAKTQLPDPLRVRLDVKDFATPVQFVNASASGDRAVISIEPSG
AFEYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGANASNTIGTPGSTSTNSPFVDMGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=708905 NK667_RS25130 WP_054616459.1 5725897..5727984(-) (pilQ) [Pseudomonas nunensis strain In5]
ATGAACAGGATTTTCTCAACCCTCGGTATTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTACAAGCGGCCACCCTCAA
CGCGCTGGATGTCGCCGCGCTGCCGGGTGACCGCGTCGAGTTGAAGTTGACCTTCGACGGGCCGCCACCACCGCCCCACG
GCTACACCACCGAATCACCGGCGCGCATCGCGCTGGATCTGCCGGGTGTAGTCAGCAAACTGACCAACAAAAACCGTGAC
CTGGGTGGCGGCAATGCCCGCAGCGCCACAGTGGTCGAGGCCAAGGACCGCACGCGACTGATCATCAACCTGACTCAACT
CACGCCCTACAGCTCCAGGGTTGAAGGCAACAATCTGTTCGTGGTCGTTGGGCAAGGCGCGAAAGGCACGACAGCCAAAC
CCGTCGCCAGCGCACCCCGCCCAGCTACCGCAAAACCGGCGCCCGCCAAGGCCTATGCGCCCGTCGGCAAGGCCATTCGC
GGCGTGGATTTTCAGCGCGGCACTCAGGGCGAAGGCAATGTGGTGATCGACTTGTCGGATCCGTCCATTGCACCGGATAT
TCAGGAGCGCGAGGGCAAGATCATCCTCGGTTTCGCCAAGACCCAATTGCCCGACCCGTTACGGGTGCGCCTGGACGTCA
AGGATTTCGCGACTCCGGTGCAGTTTGTTAACGCCAGTGCCTCCGGCGACCGCGCGGTGATCAGCATCGAGCCAAGCGGC
GCTTTCGAGTACTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCATCCGTCCGATGACCGTCGATGACTTGCAGAA
GCGCAACGCTGAGCGCTTTGCCTACACCGGCGAAAAACTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTACTGC
AACTGATCGCCGATTTCACCAACCTGAATTTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAACGTCTTGCTGGT
CGCGCCGGCGGATGAAATCGCCGCCCGTGAGCGTCAGGAACTGGAGTCGCAAAAACAGATCGCCGAACTGGCGCCGCTGC
GTCGAGAGCTGTTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCACTTCGGTGACCAGTGCCGAGGCA
AAAATCGATGAGCGGGGTTCGATCACCGTCGACGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGCCTCGA
CGAGCTTCGGCGCATCGTCGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAAGCCAACG
TCGATTACGACAAGAGTCTCGGCGTGCGCTGGGGCGGCTCGATCCAAAACAAGGGCAACTGGAACACCTCGGGTGTCAGT
AATGGCGCCAACGCGTCGAACACCATCGGCACGCCCGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGC
GGCCAACACCTCGGGGCTCGGCATCGCCTTCATCACCGACAATGTGCTGCTCGATCTTGAGCTGACGGCCATGGAAAAAA
CCGGCAACGGCGAAATCGTCTCGCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAG
ATTCCGTATCAGGAAGCCAGTTCCAGTGGTGCAACATCGGTGTCGTTCAAAGAGGCTTCGCTGTCTCTGGAAGTAACACC
GCAAATCACCCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTCAACAAAGTGCAGG
ATGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTTAACGACGGCGAGACCATCGTCATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.126

100

0.763
  comQ Acinetobacter baylyi ADP1

42.143

100

0.424
  pilQ Acinetobacter baumannii strain A118

37.769

100

0.404
  pilQ Acinetobacter baumannii D1279779

37.769

100

0.404
  pilQ Legionella pneumophila strain Lp02

37.739

97.986

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.739

97.986

0.37