Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   NK667_RS25150 Genome accession   NZ_CP101125
Coordinates   5729708..5730772 (-) Length   354 a.a.
NCBI ID   WP_054054605.1    Uniprot ID   -
Organism   Pseudomonas nunensis strain In5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5724708..5735772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK667_RS25125 (NK667_25125) aroK 5725374..5725892 (-) 519 WP_054054596.1 shikimate kinase AroK -
  NK667_RS25130 (NK667_25130) pilQ 5725897..5727984 (-) 2088 WP_054616459.1 type IV pilus secretin PilQ Machinery gene
  NK667_RS25135 (NK667_25135) - 5727998..5728525 (-) 528 WP_054054599.1 pilus assembly protein PilP -
  NK667_RS25140 (NK667_25140) pilO 5728522..5729145 (-) 624 WP_054054601.1 type 4a pilus biogenesis protein PilO -
  NK667_RS25145 (NK667_25145) - 5729142..5729708 (-) 567 WP_054616460.1 PilN domain-containing protein -
  NK667_RS25150 (NK667_25150) comM 5729708..5730772 (-) 1065 WP_054054605.1 pilus assembly protein PilM Machinery gene
  NK667_RS25155 (NK667_25155) - 5730991..5733432 (+) 2442 WP_413786161.1 penicillin-binding protein 1A -
  NK667_RS25160 (NK667_25160) - 5733538..5734716 (-) 1179 WP_054055318.1 integrase core domain-containing protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38187.70 Da        Isoelectric Point: 4.8243

>NTDB_id=708906 NK667_RS25150 WP_054054605.1 5729708..5730772(-) (comM) [Pseudomonas nunensis strain In5]
MLGLFNKKTRTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVIEKNIAELEGVGQALSRVLLKARTGIRNVAV
AVAGSAVITKTIEMDAGLSDEEMENQLKIEADQYIPYPLDEVAIDFEVQGVSVRNPERVNVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLATQLASNQERPTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTT
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=708906 NK667_RS25150 WP_054054605.1 5729708..5730772(-) (comM) [Pseudomonas nunensis strain In5]
GTGCTAGGACTCTTCAATAAAAAGACCAGAACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTTCTGGAGCT
CAGCCGCCAGGGCGACCGCTACCGGGTCGAGGCCTACGCGGTCGAACCGCTGCCGGCCAGCGCGGTCATCGAGAAAAACA
TCGCCGAGCTCGAAGGTGTGGGCCAGGCGTTGTCACGCGTGCTTCTCAAGGCTCGGACCGGTATCCGGAACGTCGCGGTT
GCGGTGGCCGGTTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGAGGAGATGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCTTATCCATTGGACGAAGTTGCCATCGATTTCGAAGTCCAGGGTGTATCGGTGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTCCGCGAGGCGGCACTGGCGCTC
GCCGGGCTGACCGCCAAGGTGGTGGACGTCGAAGCCTATGCCCTCGAACGTTCCTTTGGCCTGCTGGCGACCCAACTGGC
CTCCAATCAAGAGCGCCCGACCGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGCATAACGGCC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGCCGACAACTCACTGAAGAAATCCAGCGCCGCTATGGCCTGACCACT
GAGCAGGCCGGGCTGGCAAAGAAGCAGGGCGGTTTGCCGGACGACTATGTCAGCGAGGTGTTGCAGCCGTTTCGTGAAGC
CCTTGTGCAGCAGGTCTCTCGCTCATTGCAGTTTTTCTTTGCGTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGGCTTGATCGATTGATCGAGCAGCGGCTGGGCACGCCGACCCAGGTCGCCAACCCA
TTCGCGGACATGGCGCTGAGCAGCAAGGTCAACGCCGGCGCACTCGCCAGCGATGCCCCTGCCTTGATGATTGCCTGCGG
CCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483