Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L4174_RS02095 Genome accession   NZ_CP100425
Coordinates   451862..453544 (-) Length   560 a.a.
NCBI ID   WP_248143935.1    Uniprot ID   -
Organism   Photobacterium sp. CCB-ST2H9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446862..458544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L4174_RS02065 (L4174_002065) mutT 447626..448024 (+) 399 WP_248143929.1 8-oxo-dGTP diphosphatase MutT -
  L4174_RS02070 (L4174_002070) yacG 448078..448278 (-) 201 WP_248143930.1 DNA gyrase inhibitor YacG -
  L4174_RS02075 (L4174_002075) zapD 448286..449038 (-) 753 WP_248143931.1 cell division protein ZapD -
  L4174_RS02080 (L4174_002080) coaE 449074..449685 (-) 612 WP_248143932.1 dephospho-CoA kinase -
  L4174_RS02085 (L4174_002085) pilD 449682..450569 (-) 888 WP_248143933.1 A24 family peptidase Machinery gene
  L4174_RS02090 (L4174_002090) - 450642..451865 (-) 1224 WP_248143934.1 type II secretion system F family protein -
  L4174_RS02095 (L4174_002095) pilB 451862..453544 (-) 1683 WP_248143935.1 GspE/PulE family protein Machinery gene
  L4174_RS24075 - 453547..454038 (-) 492 WP_305885152.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L4174_RS02105 (L4174_002105) glpT 454465..455826 (+) 1362 WP_248143936.1 glycerol-3-phosphate transporter -
  L4174_RS02110 (L4174_002110) glpQ 455921..456979 (+) 1059 WP_248143937.1 glycerophosphodiester phosphodiesterase -
  L4174_RS02115 (L4174_002115) nadC 457086..457982 (-) 897 WP_248143938.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62274.33 Da        Isoelectric Point: 6.3579

>NTDB_id=705109 L4174_RS02095 WP_248143935.1 451862..453544(-) (pilB) [Photobacterium sp. CCB-ST2H9]
MTRSLLSALHQASLISQTEIDKVQHEVRQHHIGYPEALIRTGIFTDKDLARHLAHLFSLSVADVHQFDYQSLCQQLAIRP
LIERHRILPLAIDNNILTLGIADPTETSSLDECRFATGLTIQPVLLPRQQLENAIRRVYGQQVASTLNMTPVSEQDLMQL
TESPVRSDATPEDDDAPLSRYLNQLLLDAIRRQASDIHIEPFEQSCRIRFRCDGLLLIYANQPTDLAVRLTARLKILARL
NIAERRLPQDGRFRLALNQNMTVDMRVSTLPAQWGEKIVLRLLDNNHIPLSLEHLGYPDSQREAFEKALRKPQGLILVTG
PTGSGKTLSLYAGLSWLNQEHRNICTAEDPVEIQLPGINQLQINPVIGLDFSSALRTFLRQDPDVIMIGEIRDQETAAMA
VRAAQTGHLVLSTLHTNSAAETLTRLGHLGIAPYELGASLSLIISQRLVRCLCPHCRQAVSLPDSLQNHPELPITLYEAS
PQGCEYCNQGYLGRTGLFELCMSNSSAPTADNTLTMTQTPVDGLSLETAGIRLLRQGITSLSELRRVLASDPLTQLMSTA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=705109 L4174_RS02095 WP_248143935.1 451862..453544(-) (pilB) [Photobacterium sp. CCB-ST2H9]
ATGACCCGCTCCCTCCTCTCTGCGCTGCATCAGGCCAGCCTGATCTCCCAAACCGAAATCGACAAGGTTCAGCACGAAGT
CCGGCAACACCATATCGGGTATCCCGAAGCACTCATCCGGACTGGCATTTTTACCGACAAAGATCTGGCCCGGCACCTGG
CTCACCTGTTTTCATTGTCTGTAGCAGATGTCCACCAGTTTGACTATCAGAGCTTGTGCCAGCAGCTGGCTATCCGGCCG
CTGATCGAAAGACACCGGATTCTGCCGCTGGCCATCGACAACAATATTTTAACTCTGGGGATTGCAGATCCCACGGAAAC
CAGCAGCCTGGATGAGTGCCGGTTCGCAACCGGACTGACCATTCAGCCCGTGCTGCTGCCCCGGCAACAATTGGAGAATG
CGATCCGTCGCGTGTATGGTCAACAGGTTGCCAGTACTCTCAACATGACTCCCGTCAGTGAACAGGATCTGATGCAGCTG
ACCGAGTCACCGGTACGATCCGATGCCACTCCGGAAGACGATGACGCGCCGCTCAGCCGTTATTTGAACCAGCTTCTGCT
GGATGCAATCCGGCGACAGGCTTCCGACATTCACATCGAACCTTTCGAGCAATCCTGCCGGATCCGCTTTCGGTGTGATG
GATTGCTGCTAATCTATGCCAACCAGCCAACGGATTTAGCCGTCCGGCTGACTGCCCGGCTGAAAATTCTTGCCCGGCTC
AATATCGCCGAGCGCCGTCTGCCACAGGATGGCCGTTTCAGGCTGGCACTGAACCAGAACATGACCGTCGATATGCGAGT
GTCCACGCTCCCGGCACAATGGGGAGAAAAAATCGTTCTGAGACTGCTCGATAACAACCACATTCCACTCTCCCTTGAAC
ATCTGGGTTACCCGGATTCCCAGCGGGAAGCCTTCGAAAAGGCCCTGCGAAAGCCGCAAGGACTGATTCTGGTCACCGGC
CCCACTGGCAGCGGGAAAACACTGTCGCTCTATGCCGGACTGAGCTGGCTGAACCAGGAACACCGGAATATCTGTACTGC
GGAAGATCCGGTAGAGATCCAGTTGCCGGGGATCAATCAGCTCCAGATCAACCCGGTCATCGGGCTCGATTTTTCATCCG
CCTTACGTACTTTTCTCCGCCAGGATCCGGATGTCATCATGATCGGCGAGATCCGTGATCAGGAAACCGCAGCCATGGCC
GTCAGAGCTGCACAAACCGGACATCTGGTACTTTCGACGCTGCATACCAATTCTGCTGCCGAGACCCTGACCCGGCTGGG
GCATCTTGGCATCGCGCCTTATGAACTCGGCGCATCACTGAGCCTGATTATTTCCCAGCGTCTGGTGCGCTGTCTGTGCC
CCCATTGCCGGCAAGCTGTTTCCCTGCCGGATTCATTGCAAAACCATCCTGAGCTCCCGATTACCCTGTACGAAGCCAGC
CCGCAAGGCTGCGAATACTGCAATCAGGGCTATCTTGGCCGGACCGGCCTGTTCGAGCTCTGTATGTCAAATTCATCCGC
ACCAACGGCAGACAATACCCTCACCATGACCCAGACCCCGGTCGATGGCCTGTCTCTGGAAACCGCCGGTATCCGGCTGC
TGCGTCAGGGGATCACCAGCCTGAGTGAGTTACGGCGCGTCCTCGCATCTGATCCGCTCACCCAATTAATGAGCACGGCA
TGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

49.107

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.107

100

0.491
  pilB Vibrio cholerae strain A1552

49.107

100

0.491
  pilB Legionella pneumophila strain ERS1305867

41.906

99.286

0.416
  pilB Acinetobacter baumannii D1279779

40.426

100

0.407
  pilF Neisseria gonorrhoeae MS11

37.743

100

0.382
  pilB Acinetobacter baylyi ADP1

42.346

89.821

0.38
  pilB Haemophilus influenzae Rd KW20

43.841

85.536

0.375
  pilB Haemophilus influenzae 86-028NP

43.277

85

0.368