Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NFC98_RS07500 Genome accession   NZ_CP100360
Coordinates   1604686..1606467 (-) Length   593 a.a.
NCBI ID   WP_254277807.1    Uniprot ID   -
Organism   Halomonas sp. 3H     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1599686..1611467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFC98_RS07470 - 1599826..1600713 (-) 888 WP_254277459.1 DNA replication terminus site-binding protein -
  NFC98_RS07475 yacG 1600721..1600951 (-) 231 WP_183334371.1 DNA gyrase inhibitor YacG -
  NFC98_RS07480 coaE 1601040..1601651 (-) 612 WP_254277460.1 dephospho-CoA kinase -
  NFC98_RS07485 - 1601761..1602105 (+) 345 WP_254277461.1 four helix bundle protein -
  NFC98_RS07490 pilD 1602178..1603026 (-) 849 WP_254277806.1 A24 family peptidase Machinery gene
  NFC98_RS07495 pilC 1603310..1604545 (-) 1236 WP_254277462.1 type II secretion system F family protein Machinery gene
  NFC98_RS07500 pilB 1604686..1606467 (-) 1782 WP_254277807.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NFC98_RS07510 - 1606912..1607427 (+) 516 WP_367948961.1 pilin -
  NFC98_RS07515 mscL 1607674..1608105 (+) 432 WP_254277463.1 large conductance mechanosensitive channel protein MscL -
  NFC98_RS07520 - 1608198..1608743 (+) 546 WP_254277464.1 YqhA family protein -
  NFC98_RS07525 - 1608959..1610488 (-) 1530 WP_254275407.1 IS66 family transposase -
  NFC98_RS07530 tnpB 1610569..1610904 (-) 336 WP_254275406.1 IS66 family insertion sequence element accessory protein TnpB -
  NFC98_RS07535 - 1610901..1611269 (-) 369 WP_254275405.1 transposase -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 64842.30 Da        Isoelectric Point: 5.6778

>NTDB_id=704752 NFC98_RS07500 WP_254277807.1 1604686..1606467(-) (pilB) [Halomonas sp. 3H]
MNDYRTPPPHLDTPLESTRPGSASGLRGLARRLVQDGRLSEAAAARAELEAKEAEVSLLEHVIDSGLVAARPATLSSAWE
YGLPIIDLDAIRPETLPKAEAFPEKILRRLKILPLVQHGHRLTVAVPYPSTLAQLDELQFATGLTPEGVLAPVDQLGPVL
ESYLARSEKSMLDELKGVDDAIGELGYGDEGSDGEVDVQAEQNAASEDAPIIKFVNKILLDAIHRGASDIHFEPYETSYR
VRFRIDGILHDVAHPPFAMRSRISARLKVMSRLDISERRLPQDGAIKLKLSRTRSIDFRVNSLPTVYGEKVVLRILDPTS
AQLGIEQLGFTPEQKAMYETVLDQPQGMILVTGPTGSGKTVTLYTGINILNQVERNICTAEDPVEIKVPGVNQVNVLPKI
GLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSLIIAQR
LARKLCKHCKQPADIPHEVLLQQGFSEEEIANATIFEPVGCRQCTKGFKGRVGIYEVVPISEAMSNLIMKQGNSMDFDEL
ARREGHPDLRRSGLLKAMQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=704752 NFC98_RS07500 WP_254277807.1 1604686..1606467(-) (pilB) [Halomonas sp. 3H]
ATGAACGACTACCGTACCCCGCCGCCGCACCTGGACACCCCGCTGGAGAGCACGCGCCCGGGTAGTGCCAGCGGCCTGCG
CGGCCTGGCCCGCCGGCTGGTGCAGGACGGGCGGCTCTCCGAGGCGGCGGCGGCCCGCGCCGAGCTGGAGGCCAAGGAGG
CCGAGGTCTCGCTGCTGGAGCACGTGATCGACTCCGGCCTGGTGGCGGCCCGGCCGGCCACGCTCTCCTCGGCCTGGGAG
TACGGCCTGCCGATCATCGACCTGGACGCCATCCGCCCCGAGACGCTGCCCAAGGCGGAGGCCTTCCCCGAGAAGATCCT
GCGCCGGCTCAAGATCCTGCCGCTGGTGCAGCACGGCCATCGGCTCACCGTGGCGGTGCCCTACCCCTCGACGCTCGCCC
AGCTCGACGAGCTGCAGTTTGCCACCGGCCTGACCCCGGAGGGGGTACTGGCACCGGTGGACCAGCTCGGCCCGGTGCTG
GAGAGCTACCTCGCCCGCAGCGAGAAGAGCATGCTCGACGAGCTCAAGGGGGTGGACGACGCCATCGGCGAGCTGGGTTA
CGGCGACGAGGGCAGCGACGGCGAGGTGGACGTCCAGGCCGAGCAGAACGCCGCCAGCGAAGACGCGCCGATCATCAAGT
TCGTCAACAAGATCCTGCTCGACGCCATCCACCGCGGCGCCTCGGACATCCACTTCGAGCCCTACGAGACCAGCTACCGG
GTGCGCTTTCGCATCGACGGCATCCTCCACGACGTGGCTCACCCGCCCTTCGCCATGCGCTCGCGCATCTCGGCGCGCCT
CAAGGTGATGTCGCGGCTGGATATCTCCGAGCGGCGCCTGCCCCAGGACGGCGCCATCAAGCTCAAGCTCTCCCGGACCC
GCTCCATCGACTTCCGCGTCAACTCGCTGCCCACCGTCTACGGCGAGAAGGTGGTGCTGCGGATCCTCGACCCCACCTCG
GCGCAGCTAGGCATCGAGCAGCTCGGCTTCACGCCGGAACAGAAGGCGATGTACGAGACGGTGCTCGACCAGCCCCAGGG
CATGATCCTCGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGACCCTCTACACCGGCATCAACATCCTCAACCAGGTGG
AGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCCGGGGTCAACCAGGTGAACGTGCTGCCCAAGATC
GGCCTGGACTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTCGTGATGGTCGGCGAGATCCGCGACCT
GGAGACCGCCGAGATCGCCGTGAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACCGTGCACACCAACTCCGCGGCGG
AGACGCTGACGCGCCTCGCCAACATGGGCGTGGCGCCGTTCAATATCGCCAGTTCGGTGAGCCTGATCATCGCCCAGCGC
CTGGCGCGCAAGCTCTGCAAGCACTGCAAGCAGCCGGCGGACATCCCCCACGAGGTGCTGCTGCAGCAGGGCTTTTCCGA
GGAGGAGATCGCCAACGCCACCATCTTCGAGCCGGTGGGCTGCCGCCAGTGCACCAAGGGCTTCAAGGGCCGGGTGGGCA
TCTACGAGGTGGTGCCGATCTCGGAGGCGATGAGCAACCTGATCATGAAGCAGGGCAACTCCATGGACTTCGACGAGCTG
GCGAGAAGGGAGGGCCACCCGGACCTGCGCCGCAGCGGCCTGCTCAAGGCCATGCAGGGGGTGACCAGCCTGGCGGAGAT
CAACCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(217-484)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.456

96.121

0.504
  pilB Legionella pneumophila strain ERS1305867

52.456

96.121

0.504
  pilB Acinetobacter baylyi ADP1

52.281

96.121

0.503
  pilB Vibrio campbellii strain DS40M4

48.696

96.965

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

48.763

95.447

0.465
  pilB Vibrio cholerae strain A1552

50.281

89.882

0.452
  pilF Neisseria gonorrhoeae MS11

45.614

96.121

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.191

100

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

39.526

92.58

0.366