Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NKT35_RS11915 Genome accession   NZ_CP100128
Coordinates   2599281..2600987 (+) Length   568 a.a.
NCBI ID   WP_254293178.1    Uniprot ID   -
Organism   Chromobacterium sp. IIBBL 290-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2594281..2605987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKT35_RS11895 (NKT35_11895) ffh 2594916..2596271 (-) 1356 WP_254293170.1 signal recognition particle protein -
  NKT35_RS11900 (NKT35_11900) - 2596382..2597200 (+) 819 WP_254293172.1 inner membrane protein YpjD -
  NKT35_RS11905 (NKT35_11905) comE 2597296..2597574 (+) 279 WP_371926504.1 ComEA family DNA-binding protein Machinery gene
  NKT35_RS11910 (NKT35_11910) - 2597957..2599099 (+) 1143 WP_254293176.1 porin -
  NKT35_RS11915 (NKT35_11915) pilF 2599281..2600987 (+) 1707 WP_254293178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NKT35_RS11920 (NKT35_11920) pilC 2601031..2602254 (+) 1224 WP_254293180.1 type II secretion system F family protein Machinery gene
  NKT35_RS11925 (NKT35_11925) - 2602254..2603174 (+) 921 WP_254293182.1 A24 family peptidase -
  NKT35_RS11930 (NKT35_11930) coaE 2603159..2603773 (+) 615 WP_254293184.1 dephospho-CoA kinase -
  NKT35_RS11935 (NKT35_11935) zapD 2603810..2604568 (+) 759 WP_254293186.1 cell division protein ZapD -
  NKT35_RS11940 (NKT35_11940) yacG 2604574..2604765 (+) 192 WP_254293188.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62236.80 Da        Isoelectric Point: 5.8815

>NTDB_id=703713 NKT35_RS11915 WP_254293178.1 2599281..2600987(+) (pilF) [Chromobacterium sp. IIBBL 290-4]
MEASAGISGLGRALIQHNALPQQEADAIQRHAAAGNVSFVEQLILSKKMTAQEIAMFASRIFGYPLLDMSSLDVDQLPKG
LVDDDLIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEIVVVEDDKLSKVLGRYQDNSTSALKELENEDFG
DLEFADPDAQVDSGPQVEVDDAPVVKFIHKMLLDAIAGGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISKLDISEKRVPQDGRLKLVLSKSRAIDFRVSTLPTLFGEKIVMRILDSSAASLDIEQLGFEPEQKALLLSAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDLNIATAEDPVEINLPGINQVNVNEKAGLTFAFALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKKPVEIPDEALLHAGFRK
EELDGSWHPYGPVGCEECRNTGYKGRTGIYEVMPISDAMTRLIMKNGTAVDIADLAKQEGMVDLRRAGLIKVRRGLTSLA
EIEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=703713 NKT35_RS11915 WP_254293178.1 2599281..2600987(+) (pilF) [Chromobacterium sp. IIBBL 290-4]
ATGGAAGCTTCGGCAGGGATTTCCGGCCTGGGCAGGGCCCTGATACAACATAACGCGCTGCCTCAGCAAGAGGCGGACGC
CATTCAGCGCCATGCCGCGGCCGGCAATGTCAGCTTTGTCGAGCAGCTGATTCTCAGCAAGAAGATGACGGCGCAAGAGA
TCGCCATGTTCGCTTCGCGCATTTTCGGCTATCCCTTGCTCGATATGTCTTCGCTGGATGTGGATCAGTTGCCCAAGGGC
TTGGTGGATGACGATCTGATCGCCAGCCGCCGTTTGCTGCCCTTGCTGAAGCGCGGCAATAAACTGTATATCGGCACTTC
TGATCCTACCCAGACTTCGGCCTACCATGCCATTACGTTCAAGACGGGACTGACCGTGGAAATCGTGGTGGTGGAGGACG
ACAAGCTGTCCAAGGTGCTGGGCCGTTACCAGGACAACTCCACATCGGCGCTGAAGGAGTTGGAAAACGAGGACTTCGGC
GATCTCGAATTCGCCGATCCGGATGCGCAGGTCGACTCCGGACCGCAGGTGGAGGTCGATGACGCGCCGGTCGTGAAGTT
CATCCACAAGATGCTGCTGGACGCGATCGCCGGCGGCGCGTCCGACATCCATTTTGAGCCCTATGAGAAGTATTACCGCA
TCCGTTATCGGGTGGATGGCGTCTTGAAAGAGGTGGCGCAGCCGCCGCTATTGTTGAAGGAAAAAATCGCTTCGCGCATC
AAGGTGATCTCCAAGCTCGATATTTCGGAAAAGCGCGTGCCGCAGGATGGCCGGCTGAAGCTGGTGTTGTCCAAGAGCCG
GGCCATCGATTTCCGGGTCAGCACCTTGCCTACGCTGTTCGGCGAGAAAATCGTGATGCGTATCCTGGACTCGTCGGCCG
CTTCTCTGGATATCGAGCAGCTGGGCTTCGAGCCCGAGCAGAAGGCTTTGCTGCTGTCGGCCATCGCCCGGCCTTACGGC
ATGGTGCTGGTGACCGGGCCTACCGGCAGCGGCAAGACGGTGTCCTTGTATACCTGTTTGAATATCTTGAACAAGCCGGA
CCTCAACATCGCCACCGCGGAAGACCCGGTGGAAATCAATTTGCCGGGCATCAATCAGGTCAATGTCAATGAAAAGGCCG
GCCTGACCTTCGCGTTCGCGTTGAAGGCGTTTCTGCGGCAGGATCCGGATGTGATCATGGTGGGCGAGATCCGCGATTTC
GAAACCGCCGACATCGCCATCAAAGCGGCACAGACCGGGCATATGGTGTTCTCCACCCTGCACACCAATAACGCGCCGGC
GACGCTGACCCGGATGTTGAATATGGGAGTGGCGCCGTTCAATATCGCCAGTTCGGTGCTGCTGATCATGGCGCAGCGGC
TGGCGCGCCGCTTATGCAGCCATTGCAAGAAACCGGTGGAAATTCCCGATGAGGCGCTGTTGCATGCCGGCTTCCGCAAA
GAGGAGCTGGATGGCAGCTGGCATCCTTATGGTCCGGTCGGCTGCGAAGAGTGCCGCAACACCGGTTACAAGGGCAGAAC
CGGGATTTATGAAGTGATGCCGATCAGCGACGCGATGACGCGGCTGATCATGAAGAACGGCACTGCGGTGGATATCGCCG
ACCTGGCCAAGCAGGAAGGCATGGTCGATTTGCGGCGGGCGGGACTGATCAAGGTGAGGCGAGGCCTTACCTCCTTGGCG
GAGATCGAGGCGGTGACCAACGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

58.185

98.944

0.576
  pilB Acinetobacter baumannii D1279779

55.459

100

0.563
  pilB Acinetobacter baylyi ADP1

54.707

99.12

0.542
  pilB Legionella pneumophila strain ERS1305867

52.88

100

0.533
  pilB Vibrio cholerae strain A1552

50.73

96.479

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.754

98.944

0.482
  pilB Vibrio campbellii strain DS40M4

47.069

99.12

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.02

97.007

0.379
  pilF Thermus thermophilus HB27

38.188

99.12

0.379