Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NHL60_RS08395 Genome accession   NZ_CP099975
Coordinates   1762270..1763502 (-) Length   410 a.a.
NCBI ID   WP_253451381.1    Uniprot ID   -
Organism   Halomonas sp. Y3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1757270..1768502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHL60_RS08365 yacG 1757865..1758095 (-) 231 WP_253451375.1 DNA gyrase inhibitor YacG -
  NHL60_RS08370 coaE 1758092..1758733 (-) 642 WP_253451377.1 dephospho-CoA kinase -
  NHL60_RS08375 - 1758790..1759632 (-) 843 WP_301271100.1 leucine-rich repeat protein -
  NHL60_RS08385 pilD 1759748..1760599 (-) 852 WP_253451379.1 A24 family peptidase Machinery gene
  NHL60_RS08390 - 1760640..1762079 (-) 1440 WP_218314611.1 IS66 family transposase -
  NHL60_RS08395 pilC 1762270..1763502 (-) 1233 WP_253451381.1 type II secretion system F family protein Machinery gene
  NHL60_RS08400 pilB 1763508..1765277 (-) 1770 WP_253451383.1 type IV-A pilus assembly ATPase PilB -
  NHL60_RS08405 - 1765502..1766626 (+) 1125 WP_253451385.1 transposase -
  NHL60_RS19565 - 1766759..1767292 (+) 534 WP_301271101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NHL60_RS08420 - 1767596..1768030 (+) 435 WP_301271102.1 pilin -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45056.84 Da        Isoelectric Point: 10.2799

>NTDB_id=703085 NHL60_RS08395 WP_253451381.1 1762270..1763502(-) (pilC) [Halomonas sp. Y3]
MAAPTRFNDRDIKLYRWRWSGKNSRGQRVQGQLVAGSRTEVEKLLAGQRVLPTSIRRKSQIGRGLGGINSRDITLFARQM
ATMIRAGLPVLEAFQVVAESISKPAMSALVQRLLNDVAAGSTFAEALAKHPRQFDSLFVSLVAAGEQSGTLDRMLERIAF
YKEKTDSVVGRVKKALWYPTAVILIGIGVTALLLIKVVPQFESMFAGFGAELPALTQLTIRLSELAQAHWWHAILTLVGT
VLLMRLATRYSSAFAYRMHALLLRIPVIGNIVLKAVLARYARTLSITFAAGVPLIEALDTSASSTGNRVYERALREVRSS
VAEGQQLQFAMRMTDRFPAMMIQMVGIGEEAGSLDAMLERVATYYEEEVDNLVDGLTSLIEPFIIVVLGVLVGGLVLSMY
LPIFELGTVL

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=703085 NHL60_RS08395 WP_253451381.1 1762270..1763502(-) (pilC) [Halomonas sp. Y3]
ATGGCCGCTCCTACACGTTTCAATGATCGCGATATCAAGCTCTACCGCTGGCGGTGGAGCGGCAAGAACAGTCGGGGGCA
ACGGGTCCAGGGGCAGCTAGTGGCCGGCAGCCGTACAGAGGTCGAAAAGCTGCTCGCCGGTCAGCGGGTCCTGCCCACCA
GCATTCGGCGCAAGTCGCAAATAGGACGCGGCCTGGGCGGCATCAATAGCCGCGATATCACCCTGTTTGCCCGCCAGATG
GCCACCATGATCCGCGCCGGTCTGCCCGTCCTGGAGGCCTTTCAGGTGGTCGCCGAAAGTATCAGCAAACCGGCCATGAG
TGCCCTGGTGCAGCGGCTGCTCAACGACGTGGCCGCAGGCTCCACCTTCGCCGAGGCACTGGCCAAGCACCCCCGCCAGT
TCGACTCGCTGTTTGTGAGCCTGGTAGCGGCCGGCGAGCAGTCCGGCACCCTGGACCGCATGCTGGAGCGCATCGCCTTC
TACAAGGAGAAAACCGACTCGGTCGTCGGCCGAGTCAAGAAGGCGCTCTGGTACCCTACGGCCGTGATTCTGATTGGCAT
CGGCGTCACCGCCCTGCTGCTGATCAAGGTAGTGCCTCAGTTCGAGAGCATGTTCGCCGGTTTCGGCGCCGAGCTACCAG
CACTGACTCAGCTCACTATCCGACTCTCCGAGCTGGCCCAGGCCCACTGGTGGCACGCCATCCTGACCCTGGTGGGTACG
GTGCTACTGATGCGTCTGGCAACCCGCTACTCCAGCGCCTTTGCCTATCGCATGCACGCGCTGCTGCTACGTATCCCGGT
AATCGGCAATATCGTGCTCAAGGCGGTGCTGGCCCGCTACGCCCGCACCCTTTCCATCACCTTCGCCGCCGGGGTGCCGC
TGATCGAGGCCCTGGACACCTCCGCCAGCAGCACCGGCAACCGGGTCTACGAACGTGCCCTGCGCGAGGTGCGCAGCAGC
GTGGCCGAGGGCCAGCAACTGCAGTTCGCCATGCGTATGACCGACCGCTTCCCTGCCATGATGATACAGATGGTGGGCAT
CGGCGAGGAAGCCGGCTCCCTGGACGCCATGCTCGAGCGGGTGGCCACCTACTATGAGGAGGAGGTGGACAACCTGGTGG
ACGGCCTCACCTCGCTGATCGAACCCTTCATCATCGTGGTGCTGGGTGTTCTGGTAGGCGGCCTGGTGCTTTCCATGTAT
CTGCCCATCTTCGAGCTGGGCACGGTGCTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(281-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

49.132

98.293

0.483
  pilC Acinetobacter baumannii D1279779

48.615

96.829

0.471
  pilC Legionella pneumophila strain ERS1305867

48.111

96.829

0.466
  pilC Acinetobacter baylyi ADP1

47.859

96.829

0.463
  pilG Neisseria gonorrhoeae MS11

41.895

97.805

0.41
  pilG Neisseria meningitidis 44/76-A

41.895

97.805

0.41
  pilC Vibrio cholerae strain A1552

41.687

98.293

0.41
  pilC Vibrio campbellii strain DS40M4

40.399

97.805

0.395