Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF674_RS01990 Genome accession   NZ_CP099610
Coordinates   450647..452725 (+) Length   692 a.a.
NCBI ID   WP_252875464.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5VILLAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445647..457725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF674_RS01970 (NF674_01970) pilM 447862..448926 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  NF674_RS01975 (NF674_01975) - 448926..449492 (+) 567 WP_252875463.1 PilN domain-containing protein -
  NF674_RS01980 (NF674_01980) pilO 449489..450112 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  NF674_RS01985 (NF674_01985) - 450109..450633 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  NF674_RS01990 (NF674_01990) pilQ 450647..452725 (+) 2079 WP_252875464.1 type IV pilus secretin PilQ Machinery gene
  NF674_RS01995 (NF674_01995) aroK 452730..453248 (+) 519 WP_024011270.1 shikimate kinase AroK -
  NF674_RS02000 (NF674_02000) aroB 453401..454501 (+) 1101 WP_065615444.1 3-dehydroquinate synthase -
  NF674_RS02005 (NF674_02005) - 454512..456098 (+) 1587 WP_039756683.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74977.31 Da        Isoelectric Point: 5.4106

>NTDB_id=701096 NF674_RS01990 WP_252875464.1 450647..452725(+) (pilQ) [Pseudomonas moraviensis strain OTU5VILLAA1]
MNRIFSTLGFSLWIALVSPMVMAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASAPAPRPAAVASRAAPAAAAPAKFSVPRSRAIR
GVDFQRGTEGEGNVVIDLSDPIIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=701096 NF674_RS01990 WP_252875464.1 450647..452725(+) (pilQ) [Pseudomonas moraviensis strain OTU5VILLAA1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAATCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCCCAGCCCAAGG
GCTACACGACCGAGTCGCCCGCGCGTATTGCACTGGATCTGCCGGGGGTGACCAGCCAGCTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGCAATGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCATACAGCACGCGGGTCGAAGGCAATAATCTGTTCGTGGTGGTTGGTCAGGGCGCCTCCGCGCCCGCGCCGCGCC
CGGCCGCCGTTGCATCACGTGCCGCCCCGGCCGCTGCGGCGCCCGCGAAATTTTCTGTGCCGCGCAGCCGGGCAATTCGC
GGCGTCGATTTCCAGCGCGGTACCGAGGGCGAAGGCAACGTGGTCATCGATCTCTCTGATCCGATCATCGCCCCGGATAT
CCAGGAACACGACGGCAAGATCATCCTCAGCTTCGCCCGCACCCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCGACCTTCCAGACTGATAACAAGCTCACGGTGAGCGTTCGCCCGATGAGTGTCGACGATCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGTGACAAGCTGTCGCTGAATTTTCAGGACATCGACGTACGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAACCTCAACCTCGTGGCAAGCGACACGGTGCAGGGCGGTATCACGTTGCGCCTGCAGAAC
GTGCCGTGGGACCAAGCACTGGATCTGGTGTTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTATTGGT
GGCGCCGGCCGATGAAATCGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCATTGC
GTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCGGACATTGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATCACTGTCGACGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAATTACGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTCGGCGTACGCTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGCTCATCGAGCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCCTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAGATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATCCCGTAT
CAGGAGGCCAGCTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAAGCTTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.667

100

0.746
  comQ Acinetobacter baylyi ADP1

41.04

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.038

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.038

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.683

98.555

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.647

98.266

0.37