Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NF674_RS01970 Genome accession   NZ_CP099610
Coordinates   447862..448926 (+) Length   354 a.a.
NCBI ID   WP_042608693.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5VILLAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442862..453926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF674_RS01960 (NF674_01960) - 443610..444878 (+) 1269 WP_042608691.1 malic enzyme-like NAD(P)-binding protein -
  NF674_RS01965 (NF674_01965) - 445198..447645 (-) 2448 WP_042608692.1 penicillin-binding protein 1A -
  NF674_RS01970 (NF674_01970) pilM 447862..448926 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  NF674_RS01975 (NF674_01975) - 448926..449492 (+) 567 WP_252875463.1 PilN domain-containing protein -
  NF674_RS01980 (NF674_01980) pilO 449489..450112 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  NF674_RS01985 (NF674_01985) - 450109..450633 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  NF674_RS01990 (NF674_01990) pilQ 450647..452725 (+) 2079 WP_252875464.1 type IV pilus secretin PilQ Machinery gene
  NF674_RS01995 (NF674_01995) aroK 452730..453248 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38062.62 Da        Isoelectric Point: 4.8225

>NTDB_id=701095 NF674_RS01970 WP_042608693.1 447862..448926(+) (pilM) [Pseudomonas moraviensis strain OTU5VILLAA1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGCKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVTEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=701095 NF674_RS01970 WP_042608693.1 447862..448926(+) (pilM) [Pseudomonas moraviensis strain OTU5VILLAA1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGTCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGTGTCGGCCAGGCGCTCAGCCGGTTGCTGGTCAAGGCACGCACCGGGTGCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCGCTGGCGCTC
GCCGGCCTGACTGCACGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGGCTGCTGGCGACGCATCTGGC
TGCTTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGCGCGACGATGACCACCCTCAGCGTCCTGCATAACGGCA
AGATCATCTATACCCGCGAGCAACTCTTTGGCGGGCGCCAGCTCACCGAAGAGATCCAGCGCCGCTATGGCCTCACTGTC
GAGCAGGCGGGCCTGGCGAAAAAGCAGGGCGGCCTGCCTGACGATTACGTGACCGAAGTCCTGCAGCCATTTCGCGAGGC
ACTGGTGCAGCAGGTTTCGCGTTCGCTGCAGTTCTTCTTCGCCTCTGGCCAGTACAACGCCGTCGACCATATCCTGCTGG
CCGGCGGCACGGCCTCTGTACCCGGGCTGGATCGCCTGATCGAACAGCGCTTGAACACGCCGACTCAGGTTGCCAACCCG
TTTGCCGACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCCGCATTGATGATCGCCTGTGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48