Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF673_RS02020 Genome accession   NZ_CP099609
Coordinates   456133..458211 (+) Length   692 a.a.
NCBI ID   WP_252881172.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5MASSA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451133..463211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF673_RS02000 (NF673_02000) pilM 453348..454412 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  NF673_RS02005 (NF673_02005) - 454412..454978 (+) 567 WP_042608694.1 PilN domain-containing protein -
  NF673_RS02010 (NF673_02010) pilO 454975..455598 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  NF673_RS02015 (NF673_02015) - 455595..456119 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  NF673_RS02020 (NF673_02020) pilQ 456133..458211 (+) 2079 WP_252881172.1 type IV pilus secretin PilQ Machinery gene
  NF673_RS02025 (NF673_02025) aroK 458216..458734 (+) 519 WP_027611316.1 shikimate kinase AroK -
  NF673_RS02030 (NF673_02030) aroB 458887..459987 (+) 1101 WP_252881173.1 3-dehydroquinate synthase -
  NF673_RS02035 (NF673_02035) - 459998..461584 (+) 1587 WP_252881174.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74920.08 Da        Isoelectric Point: 5.4002

>NTDB_id=701067 NF673_RS02020 WP_252881172.1 456133..458211(+) (pilQ) [Pseudomonas moraviensis strain OTU5MASSA1]
MNRIFSTLGFSLWIALVSPMVMAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATATSAPARSAAPRSRSIH
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=701067 NF673_RS02020 WP_252881172.1 456133..458211(+) (pilQ) [Pseudomonas moraviensis strain OTU5MASSA1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAACCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCCCAGCCCAAGG
GCTACACGACCGAGTCGCCCGCGCGTATTGCACTGGATCTGCCGGGGGTGGCCAGCCAGTTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGCAATGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCATACAGCACGCGGGTCGAAGGCAATAATCTGTTCGTGGTGGTTGGTCAGGGCGCCTCCGCGACCGCGCCGCGCC
CGGCCGCCGTTGCACCACGTGCCGCGACGGCGACTTCGGCGCCCGCCAGATCCGCGGCGCCGCGCAGCCGGTCGATTCAC
GGCGTTGATTTCCAGCGCGGCACCGAAGGCGAGGGCAACGTGGTCATCGACCTCTCTGACCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCTCGCACGCAATTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AAGACTTCGCCACACCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCGACCTTCCAGACTGATAACAAGCTCACGGTGAGCGTTCGCCCGATGAGTGTCGACGATCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTGTCGCTGAATTTTCAGGACATCGATGTACGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAACCTCGTGGCAAGCGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGTTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTACTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCATTGC
GTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCGGACATTGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAATTACGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTCGGCGTACGCTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGCTCATCGAGCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCCTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTGGAGCTTACGGCGATGGAAAAGACCGGCAACG
GCGAGATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATACCGTAT
CAGGAGGCCAGCTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.927

100

0.744
  comQ Acinetobacter baylyi ADP1

41.474

100

0.415
  pilQ Acinetobacter baumannii strain A118

38.306

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.306

100

0.412
  pilQ Legionella pneumophila strain Lp02

37.85

98.121

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.832

0.37