Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF679_RS01910 Genome accession   NZ_CP099601
Coordinates   433761..435848 (+) Length   695 a.a.
NCBI ID   WP_252868654.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MONEA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428761..440848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF679_RS01890 (NF679_01890) pilM 430976..432040 (+) 1065 WP_252868653.1 pilus assembly protein PilM Machinery gene
  NF679_RS01895 (NF679_01895) - 432040..432606 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF679_RS01900 (NF679_01900) pilO 432603..433226 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  NF679_RS01905 (NF679_01905) - 433223..433747 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF679_RS01910 (NF679_01910) pilQ 433761..435848 (+) 2088 WP_252868654.1 type IV pilus secretin PilQ Machinery gene
  NF679_RS01915 (NF679_01915) aroK 435853..436371 (+) 519 WP_024011270.1 shikimate kinase AroK -
  NF679_RS01920 (NF679_01920) aroB 436524..437624 (+) 1101 WP_073471604.1 3-dehydroquinate synthase -
  NF679_RS01925 (NF679_01925) - 437635..439221 (+) 1587 WP_252868655.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75150.43 Da        Isoelectric Point: 5.4106

>NTDB_id=700979 NF679_RS01910 WP_252868654.1 433761..435848(+) (pilQ) [Pseudomonas siliginis strain OTU6MONEA1]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLTNKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATAATAAAAPARPAAQRSR
AIRGVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVE
PSGTFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNAS
GVNGSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=700979 NF679_RS01910 WP_252868654.1 433761..435848(+) (pilQ) [Pseudomonas siliginis strain OTU6MONEA1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGACCGCGTCGAATTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGCTGACCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGCAGCAGG
GCGATTCGCGGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAATGTGGTCATCGATCTCTCTGATCCGACCATCGC
CCCGGATATCCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCC
TCGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAG
CCCAGCGGCACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGGCCGATGAGCATCGACGA
CCTGCAAAAGCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGTT
CGGTACTGCAACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACTTTGCGC
CTGCAGAACGTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGT
GCTGCTGGTCGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGG
CGCCCCTGCGTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGA
TCGCCTCGATGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTG
AGGCCAACGTCGATTATGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCGTCC
GGGGTCAATGGCTCCTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGT
CAACAATACCTCGGGGATCGGCATCGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAACTGACGGCGATGGAAAAGA
CCGGCAACGGTGAAATCGTCTCGCAGCCGAAGGTAGTCACCTCGGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAG
ATTCCCTATCAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCC
GCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAAACCATCGTAATCGGCGGTGTT
TTTTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.827

100

0.741
  comQ Acinetobacter baylyi ADP1

41.32

100

0.414
  pilQ Acinetobacter baumannii D1279779

37.5

100

0.401
  pilQ Acinetobacter baumannii strain A118

37.5

100

0.401
  pilQ Legionella pneumophila strain ERS1305867

38.088

97.842

0.373
  pilQ Legionella pneumophila strain Lp02

37.83

98.129

0.371