Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NF679_RS01890 Genome accession   NZ_CP099601
Coordinates   430976..432040 (+) Length   354 a.a.
NCBI ID   WP_252868653.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MONEA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425976..437040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF679_RS01880 (NF679_01880) - 426723..427991 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  NF679_RS01885 (NF679_01885) - 428312..430759 (-) 2448 WP_056787328.1 penicillin-binding protein 1A -
  NF679_RS01890 (NF679_01890) pilM 430976..432040 (+) 1065 WP_252868653.1 pilus assembly protein PilM Machinery gene
  NF679_RS01895 (NF679_01895) - 432040..432606 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF679_RS01900 (NF679_01900) pilO 432603..433226 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  NF679_RS01905 (NF679_01905) - 433223..433747 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF679_RS01910 (NF679_01910) pilQ 433761..435848 (+) 2088 WP_252868654.1 type IV pilus secretin PilQ Machinery gene
  NF679_RS01915 (NF679_01915) aroK 435853..436371 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38033.52 Da        Isoelectric Point: 4.7453

>NTDB_id=700978 NF679_RS01890 WP_252868653.1 430976..432040(+) (pilM) [Pseudomonas siliginis strain OTU6MONEA1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYSVEPLPANAVVEKNIADLEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPDRVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=700978 NF679_RS01890 WP_252868653.1 430976..432040(+) (pilM) [Pseudomonas siliginis strain OTU6MONEA1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATTCGGTGGAGCCGTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGACCTCGAAGGCGTCGGCCAGGCGCTGAGCCGGTTGCTGGTGAAAGCGCGAACCGGGGTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGATCGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGACGTCGAGGCCTACGCCCTCGAGCGCTCGTTCGGTCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGTGCGACGATGACCACCCTCAGCGTGCTGCACAACGGCA
AGATCATCTACACCCGTGAGCAATTATTCGGCGGACGCCAGCTTACCGAGGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGTTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGGCTGGACACGCCGACGCAGGTCGCCAACCCC
TTCGCCGACATGGCCTTGGGCAGTAAGGTCAACGCAGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486