Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF678_RS01900 Genome accession   NZ_CP099600
Coordinates   441668..443746 (+) Length   692 a.a.
NCBI ID   WP_150638039.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MEDAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436668..448746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF678_RS01880 (NF678_01880) comM 438883..439947 (+) 1065 WP_252883035.1 pilus assembly protein PilM Machinery gene
  NF678_RS01885 (NF678_01885) - 439947..440513 (+) 567 WP_252883036.1 PilN domain-containing protein -
  NF678_RS01890 (NF678_01890) pilO 440510..441133 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  NF678_RS01895 (NF678_01895) - 441130..441654 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF678_RS01900 (NF678_01900) pilQ 441668..443746 (+) 2079 WP_150638039.1 type IV pilus secretin PilQ Machinery gene
  NF678_RS01905 (NF678_01905) aroK 443751..444269 (+) 519 WP_024011270.1 shikimate kinase AroK -
  NF678_RS01910 (NF678_01910) aroB 444422..445522 (+) 1101 WP_056787345.1 3-dehydroquinate synthase -
  NF678_RS01915 (NF678_01915) - 445533..447119 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74907.17 Da        Isoelectric Point: 5.4106

>NTDB_id=700949 NF678_RS01900 WP_150638039.1 441668..443746(+) (pilQ) [Pseudomonas siliginis strain OTU6MEDAA1]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLTNKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAVAAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=700949 NF678_RS01900 WP_150638039.1 441668..443746(+) (pilQ) [Pseudomonas siliginis strain OTU6MEDAA1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGACCGCGTCGAATTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGCTGACCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCCGTCGCTGCACCGCGTGCCGCCACGGCCGCCGCAGCACCGGCCAGACCGGCCGCGCAACGCAGCAGGGCGATTCGC
GGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAATGTGGTCATCGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGTCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAACCTGGTGGCGAGCGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
TGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTATGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCGTCCGGGGTCAAT
GGCTCCTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATCGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCGAAGGTAGTCACCTCGGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACACCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGCGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii D1279779

37.787

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.787

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.997

98.121

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.832

0.371