Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF677_RS01910 Genome accession   NZ_CP099599
Coordinates   441389..443467 (+) Length   692 a.a.
NCBI ID   WP_150638039.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436389..448467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS01890 (NF677_01890) pilM 438604..439668 (+) 1065 WP_150741723.1 pilus assembly protein PilM Machinery gene
  NF677_RS01895 (NF677_01895) - 439668..440234 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF677_RS01900 (NF677_01900) pilO 440231..440854 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  NF677_RS01905 (NF677_01905) - 440851..441375 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF677_RS01910 (NF677_01910) pilQ 441389..443467 (+) 2079 WP_150638039.1 type IV pilus secretin PilQ Machinery gene
  NF677_RS01915 (NF677_01915) aroK 443472..443990 (+) 519 WP_024011270.1 shikimate kinase AroK -
  NF677_RS01920 (NF677_01920) aroB 444143..445243 (+) 1101 WP_252885179.1 3-dehydroquinate synthase -
  NF677_RS01925 (NF677_01925) - 445254..446840 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74907.17 Da        Isoelectric Point: 5.4106

>NTDB_id=700919 NF677_RS01910 WP_150638039.1 441389..443467(+) (pilQ) [Pseudomonas siliginis strain OTU6ESPEB1]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLTNKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAVAAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=700919 NF677_RS01910 WP_150638039.1 441389..443467(+) (pilQ) [Pseudomonas siliginis strain OTU6ESPEB1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGACCGCGTCGAATTGAAGCTGTCGTTCGACGGCCCGCCACCGCAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGCTGACCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCCGTCGCTGCACCGCGTGCCGCCACGGCCGCCGCAGCACCGGCCAGACCGGCCGCGCAACGCAGCAGGGCGATTCGC
GGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAATGTGGTCATCGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGTCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAACCTGGTGGCGAGCGACACGGTGCAGGGCGGTATCACCTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGTTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACGAGCGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
TGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTATGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCGTCCGGGGTCAAT
GGCTCCTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATCGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCGAAGGTAGTCACCTCGGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii D1279779

37.787

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.787

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.997

98.121

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.832

0.371