Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NF677_RS01890 Genome accession   NZ_CP099599
Coordinates   438604..439668 (+) Length   354 a.a.
NCBI ID   WP_150741723.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6ESPEB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433604..444668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF677_RS01880 (NF677_01880) - 434351..435619 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  NF677_RS01885 (NF677_01885) - 435940..438387 (-) 2448 WP_095181031.1 penicillin-binding protein 1A -
  NF677_RS01890 (NF677_01890) pilM 438604..439668 (+) 1065 WP_150741723.1 pilus assembly protein PilM Machinery gene
  NF677_RS01895 (NF677_01895) - 439668..440234 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF677_RS01900 (NF677_01900) pilO 440231..440854 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  NF677_RS01905 (NF677_01905) - 440851..441375 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF677_RS01910 (NF677_01910) pilQ 441389..443467 (+) 2079 WP_150638039.1 type IV pilus secretin PilQ Machinery gene
  NF677_RS01915 (NF677_01915) aroK 443472..443990 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38043.55 Da        Isoelectric Point: 4.7453

>NTDB_id=700918 NF677_RS01890 WP_150741723.1 438604..439668(+) (pilM) [Pseudomonas siliginis strain OTU6ESPEB1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIADLEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPDRVNVLLAACRKENVEVREAALAL
AGLTPRVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=700918 NF677_RS01890 WP_150741723.1 438604..439668(+) (pilM) [Pseudomonas siliginis strain OTU6ESPEB1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCGTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGACCTCGAAGGCGTCGGCCAGGCGCTGAGCCGGTTGCTGGTGAAAGCGCGAACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGATCGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTACGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCCCGCGGGTGGTGGACGTCGAGGCCTACGCCCTCGAGCGCTCGTTCGGTCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGTGCGACCATGACCACTCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGAGAGCAATTGTTCGGCGGACGCCAGCTCACCGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCTCCGGGCAGTACAACGCGGTCGACCATATCCTGTTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGGCTGGACACGCCGACGCAGGTCGCCAACCCA
TTCGCCGACATGGCCTTGGGCAGCAAGGTCAACGCAGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489