Detailed information NTG0700647

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCP45_RS11470
• Genome accession: NZ_CP115270
• Coordinates: 2401912..2402976 (-)
• Length: 354 a.a.
• NCBI ID: WP_034074518.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2396912..2407976

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP45_RS11445 (PCP45_11450)	aroK	2397447..2397965 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PCP45_RS11450 (PCP45_11455)	pilQ	2397977..2400121 (-)	2145	WP_329038454.1	type 4a pilus secretin PilQ	Machinery gene
PCP45_RS11455 (PCP45_11460)	comL/pilP	2400175..2400699 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PCP45_RS11460 (PCP45_11465)	pilO	2400696..2401319 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
PCP45_RS11465 (PCP45_11470)	pilN	2401316..2401912 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
PCP45_RS11470 (PCP45_11475)	pilM	2401912..2402976 (-)	1065	WP_034074518.1	type IV pilus assembly protein PilM	Machinery gene
PCP45_RS11475 (PCP45_11480)	-	2403161..2405632 (+)	2472	WP_058169786.1	penicillin-binding protein 1A	-
PCP45_RS11480 (PCP45_11485)	-	2405728..2406996 (-)	1269	WP_003095842.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37968.51 Da
• Isoelectric Point: 4.5405

>NTDB_id=700647 PCP45_RS11470 WP_034074518.1 2401912..2402976(-) (pilM) [Pseudomonas aeruginosa strain F019]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGLALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=700647 PCP45_RS11470 WP_034074518.1 2401912..2402976(-) (pilM) [Pseudomonas aeruginosa strain F019]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCTGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGATGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGATTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466