Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP45_RS11460 Genome accession   NZ_CP115270
Coordinates   2400696..2401319 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395696..2406319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP45_RS11440 (PCP45_11445) aroB 2396291..2397397 (-) 1107 WP_080911875.1 3-dehydroquinate synthase -
  PCP45_RS11445 (PCP45_11450) aroK 2397447..2397965 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PCP45_RS11450 (PCP45_11455) pilQ 2397977..2400121 (-) 2145 WP_329038454.1 type 4a pilus secretin PilQ Machinery gene
  PCP45_RS11455 (PCP45_11460) comL/pilP 2400175..2400699 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP45_RS11460 (PCP45_11465) pilO 2400696..2401319 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP45_RS11465 (PCP45_11470) pilN 2401316..2401912 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP45_RS11470 (PCP45_11475) pilM 2401912..2402976 (-) 1065 WP_034074518.1 type IV pilus assembly protein PilM Machinery gene
  PCP45_RS11475 (PCP45_11480) - 2403161..2405632 (+) 2472 WP_058169786.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=700645 PCP45_RS11460 WP_003103268.1 2400696..2401319(-) (pilO) [Pseudomonas aeruginosa strain F019]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=700645 PCP45_RS11460 WP_003103268.1 2400696..2401319(-) (pilO) [Pseudomonas aeruginosa strain F019]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTT
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401