Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NGM19_RS01975 Genome accession   NZ_CP099548
Coordinates   430982..432706 (-) Length   574 a.a.
NCBI ID   WP_125318237.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ6079     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425982..437706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM19_RS01945 (NGM19_01945) - 426066..426392 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  NGM19_RS01950 (NGM19_01950) rlmB 426582..427331 (+) 750 WP_151711193.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NGM19_RS01955 (NGM19_01955) - 427362..428273 (+) 912 WP_151711192.1 DMT family transporter -
  NGM19_RS01960 (NGM19_01960) coaE 428270..428869 (-) 600 WP_151711191.1 dephospho-CoA kinase -
  NGM19_RS01965 (NGM19_01965) pilD 428871..429731 (-) 861 WP_151711190.1 A24 family peptidase Machinery gene
  NGM19_RS01970 (NGM19_01970) pilC 429731..430957 (-) 1227 WP_125318236.1 type II secretion system F family protein Machinery gene
  NGM19_RS01975 (NGM19_01975) pilB 430982..432706 (-) 1725 WP_125318237.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGM19_RS01980 (NGM19_01980) tpiA 433052..433843 (+) 792 WP_004958617.1 triose-phosphate isomerase -
  NGM19_RS01985 (NGM19_01985) secG 433856..434185 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  NGM19_RS02005 (NGM19_02005) rimP 434808..435332 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  NGM19_RS02010 (NGM19_02010) nusA 435370..436854 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63595.75 Da        Isoelectric Point: 5.1320

>NTDB_id=700600 NGM19_RS01975 WP_125318237.1 430982..432706(-) (pilB) [Acinetobacter junii strain AJ6079]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSLL
EMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQ
GITSLQEVNRVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=700600 NGM19_RS01975 WP_125318237.1 430982..432706(-) (pilB) [Acinetobacter junii strain AJ6079]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCCTTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATATTAAACACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TCGTGATTGCTCAACGTTTAGCCCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTTCTA
GAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATGGGA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.076

100

0.814
  pilB Acinetobacter baylyi ADP1

76.348

100

0.765
  pilB Legionella pneumophila strain ERS1305867

52.974

93.728

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

97.038

0.483
  pilF Neisseria gonorrhoeae MS11

50.274

95.296

0.479
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

50.87

90.07

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.253

100

0.368