Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NG827_RS05670 Genome accession   NZ_CP099533
Coordinates   1332456..1334189 (+) Length   577 a.a.
NCBI ID   WP_267098129.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain LT6-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1327456..1339189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG827_RS05650 (NG827_05640) coaE 1328606..1329235 (-) 630 WP_267098125.1 dephospho-CoA kinase -
  NG827_RS05655 (NG827_05645) - 1329249..1330112 (-) 864 WP_267098126.1 A24 family peptidase -
  NG827_RS05660 (NG827_05650) pilC 1330119..1331378 (-) 1260 WP_267098127.1 type II secretion system F family protein Machinery gene
  NG827_RS05665 (NG827_05655) - 1331737..1332177 (+) 441 WP_267098128.1 pilin -
  NG827_RS05670 (NG827_05660) pilB 1332456..1334189 (+) 1734 WP_267098129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NG827_RS05675 (NG827_05665) pilR 1334420..1335808 (-) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator
  NG827_RS05680 (NG827_05670) - 1335891..1337504 (-) 1614 WP_267098130.1 ATP-binding protein -
  NG827_RS05685 (NG827_05675) sucC 1337896..1339065 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62597.87 Da        Isoelectric Point: 5.6445

>NTDB_id=700451 NG827_RS05670 WP_267098129.1 1332456..1334189(+) (pilB) [Xanthomonas sacchari strain LT6-2]
MNTAVAANLVGITGIARRLVQDGALEEAAARTAMAQAAEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGGGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=700451 NG827_RS05670 WP_267098129.1 1332456..1334189(+) (pilB) [Xanthomonas sacchari strain LT6-2]
ATGAATACAGCTGTGGCAGCCAATTTGGTCGGCATCACCGGGATCGCCCGCCGCCTCGTGCAGGACGGTGCGCTGGAGGA
AGCCGCGGCGCGGACCGCCATGGCCCAGGCGGCCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCCGCGCAGCTGGCCGCCGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTCTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCGGCGGTGGCGACACCGGCATCGACGC
CAAGGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTGGATGCGATCCGCCGCGGCGCGTCCGACATCC
ACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGGTCCCACAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGAACAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGTTATTCGGCGAGAAGA
TCGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCCGAGCAGCAGAAGCTG
TTCCTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACGGTGTCGCTGTA
CACGGCGCTGGGCATCCTCAACGACGACACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGTCTGCCCGGTG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACCCTGCACACCAACGACGCGCCGCAGACCATTGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGTCCGGTCCAATTGCCGGAA
AAGGCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAAGCGGTCGGCTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGC
AGGGCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCCCTGCTCAAG
GCCCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.72

99.133

0.542
  pilB Acinetobacter baumannii D1279779

54.912

98.787

0.542
  pilB Legionella pneumophila strain ERS1305867

50.78

100

0.508
  pilB Vibrio cholerae strain A1552

49.913

99.307

0.496
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.557

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.037

100

0.381
  pilF Thermus thermophilus HB27

39.619

90.988

0.36