Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L1F06_RS02900 Genome accession   NZ_CP099397
Coordinates   614376..616445 (+) Length   689 a.a.
NCBI ID   WP_177491049.1    Uniprot ID   -
Organism   Pseudomonas hydrolytica strain DSWY01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 609376..621445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F06_RS02880 (L1F06_002880) comM 611544..612608 (+) 1065 WP_003246678.1 pilus assembly protein PilM Machinery gene
  L1F06_RS02885 (L1F06_002885) pilN 612608..613180 (+) 573 WP_129481890.1 type 4a pilus biogenesis protein PilN -
  L1F06_RS02890 (L1F06_002890) pilO 613177..613797 (+) 621 WP_129481889.1 type 4a pilus biogenesis protein PilO -
  L1F06_RS02895 (L1F06_002895) pilP 613797..614321 (+) 525 WP_129481888.1 type 4a pilus biogenesis lipoprotein PilP -
  L1F06_RS02900 (L1F06_002900) pilQ 614376..616445 (+) 2070 WP_177491049.1 type IV pilus secretin PilQ Machinery gene
  L1F06_RS02905 (L1F06_002905) aroK 616451..616969 (+) 519 WP_003246673.1 shikimate kinase AroK -
  L1F06_RS02910 (L1F06_002910) aroB 617145..618248 (+) 1104 WP_011920765.1 3-dehydroquinate synthase -
  L1F06_RS02915 (L1F06_002915) - 618259..619836 (+) 1578 WP_129481886.1 AAA family ATPase -

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74275.23 Da        Isoelectric Point: 4.8061

>NTDB_id=699470 L1F06_RS02900 WP_177491049.1 614376..616445(+) (pilQ) [Pseudomonas hydrolytica strain DSWY01]
MKSSLSRIGVVLLLAMSSPMVLAANLQALDVASLPGDRVELRLTFDEPVSAPRGYTIEQPARIALDLPGVTNKLGAKNRE
LGVGNARSVTIVEAKDRTRLIVNLTNLAPYSTRVEGNNLYVLVGAGTVANTPAVAPVAVAPVSGGHAVSAGKSVSNIDFQ
RGEQGEGNVFITLSDATVTPNIQEQGGKIRLDFAKTQLPDSLRVRLDVKDFATPVQFVSATHVSGSASIIVEPVGLYDYL
AYQTENKLTLSVKPLSQDDADRRKAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDIATLFQSVANIEEGGSER
GSVTVDDRTNSIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYEKALGVRWGGTKVFGGGKWVGYGNDDNGD
ESGNSGDEASGNFPFVDLGVTGRTSGIGIGFVTDNTILDLELSAMEKTGNGEIVSQPKVVTADKETAKILKGTEVPYQES
SSSGATTVSFKEAALALEVTPQITPDNRIIIEVKVNKDEPDYTNAVLGVPPIKKNEVNAKVLVSDGETIVIGGVYSNTQS
KAVDKVPFLGDLPYLGRVFRRDVVTDQKSELLIFLTPRILNTQAIAVNR

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=699470 L1F06_RS02900 WP_177491049.1 614376..616445(+) (pilQ) [Pseudomonas hydrolytica strain DSWY01]
ATGAAGAGCTCTTTGTCGCGTATAGGGGTCGTGTTGCTGCTTGCTATGTCTTCGCCTATGGTGTTGGCGGCAAACTTACA
AGCTCTCGATGTAGCAAGTCTCCCAGGTGATCGCGTTGAGCTCAGGCTAACGTTTGATGAGCCGGTATCGGCTCCTCGAG
GTTACACAATCGAACAGCCGGCGCGGATCGCGCTTGATTTACCAGGTGTGACCAACAAGCTCGGTGCCAAGAATCGTGAG
CTTGGCGTGGGGAACGCTCGCAGTGTAACGATTGTGGAGGCCAAGGATCGGACTCGCTTGATAGTCAATCTCACTAATTT
AGCGCCTTATAGTACTCGGGTAGAGGGCAATAATCTCTATGTGCTGGTTGGGGCGGGGACTGTCGCTAATACACCGGCGG
TTGCGCCAGTGGCTGTTGCGCCGGTGTCTGGTGGTCATGCTGTCAGCGCCGGAAAATCTGTAAGTAATATAGATTTTCAG
CGTGGGGAGCAGGGGGAGGGTAATGTTTTCATTACTCTTTCCGATGCAACGGTAACGCCTAACATACAAGAGCAGGGTGG
AAAAATCCGCCTCGATTTTGCCAAGACGCAATTGCCCGATTCTTTAAGGGTGCGGCTGGATGTTAAAGATTTTGCGACTC
CTGTGCAGTTTGTCAGTGCTACGCACGTTTCGGGGAGTGCAAGTATCATTGTCGAGCCTGTGGGTCTATATGATTATTTG
GCGTATCAAACGGAAAATAAGCTAACGCTCAGTGTGAAGCCGTTGAGTCAAGATGACGCTGATCGGCGCAAGGCAGAGAG
ATTTGCCTATACGGGCGAGAAGCTGTCGCTCAATTTTCAGGATATTGATGTTCGTTCCGTTCTGCAGTTGATCGCTGATT
TTACGGATCTGAATCTTGTTGCCAGTGATACTGTTGCAGGAAATATTACCCTGCGTTTGCAAAACGTTCCTTGGGACCAG
GCGCTTGATCTGGTCTTGAAGACCAAGGGGCTAGATAAGCGAAAAGTTGGTAACGTCCTACTCGTCGCGCCCGCTGATGA
GATAGCTGCGCGCGAGCGCCAGGAGCTGGAGTCTCAGAAGCAGATTGCGGAGTTGGCCCCGCTGCGCAGAGAGTTGATTC
AGGTTAACTATGCGAAGGCTTCAGATATAGCTACGCTCTTCCAATCGGTAGCTAATATTGAAGAGGGCGGCAGCGAGCGA
GGTTCTGTTACTGTCGACGATCGAACCAATAGCATCATCGCCTATCAGACTCAAGAGCGTTTGGATGAGCTCAGAAGAAT
TGTTGCTCAATTGGATATTCCAGTTCGCCAGGTAATGATTGAGGCCAGAATAGTTGAGGCCAATGTCGATTATGAGAAAG
CTCTTGGCGTGCGCTGGGGGGGGACTAAGGTATTTGGCGGGGGTAAGTGGGTTGGCTATGGTAACGACGATAACGGGGAT
GAAAGCGGGAACTCTGGGGATGAGGCAAGTGGTAACTTTCCCTTCGTTGATCTAGGGGTGACGGGGCGAACGTCGGGAAT
TGGTATCGGTTTTGTAACGGATAATACCATTCTAGATCTTGAGCTTTCTGCGATGGAAAAAACAGGGAATGGCGAGATTG
TCTCTCAGCCGAAAGTCGTGACGGCCGATAAGGAAACGGCGAAGATCCTTAAGGGGACCGAGGTGCCATACCAAGAGTCC
AGTTCGAGCGGTGCTACAACCGTTTCTTTCAAGGAGGCGGCTTTGGCTCTGGAGGTGACGCCTCAAATTACCCCTGATAA
TCGGATTATTATCGAGGTTAAAGTCAATAAGGACGAGCCGGATTACACAAATGCTGTGCTTGGTGTACCGCCTATCAAGA
AGAATGAGGTTAACGCAAAAGTCCTCGTGTCAGACGGTGAGACGATTGTTATAGGCGGCGTTTATTCCAATACGCAATCC
AAGGCTGTTGACAAAGTTCCGTTCTTGGGAGACCTGCCCTATCTTGGCCGCGTATTCCGTCGCGATGTGGTCACCGACCA
AAAGTCGGAGCTGCTTATCTTCTTGACTCCGCGTATTCTGAACACTCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(524-680)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.095

100

0.772
  pilQ Acinetobacter baumannii strain A118

39.728

100

0.424
  pilQ Acinetobacter baumannii D1279779

39.728

100

0.424
  comQ Acinetobacter baylyi ADP1

41.453

100

0.422
  pilQ Legionella pneumophila strain ERS1305867

38.074

97.968

0.373
  pilQ Legionella pneumophila strain Lp02

38.074

97.968

0.373