Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NFG60_RS18795 Genome accession   NZ_CP098772
Coordinates   4427381..4428493 (-) Length   370 a.a.
NCBI ID   WP_252118958.1    Uniprot ID   -
Organism   Alteromonas macleodii strain QZ9-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4422381..4433493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFG60_RS18770 (NFG60_18770) - 4423937..4424665 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFG60_RS18775 (NFG60_18775) gshB 4424804..4425757 (+) 954 WP_014950459.1 glutathione synthase -
  NFG60_RS18780 (NFG60_18780) - 4425886..4426452 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  NFG60_RS18785 (NFG60_18785) ruvX 4426445..4426876 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  NFG60_RS18790 (NFG60_18790) - 4426923..4427240 (-) 318 WP_039227917.1 DUF2007 domain-containing protein -
  NFG60_RS18795 (NFG60_18795) pilU 4427381..4428493 (-) 1113 WP_252118958.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NFG60_RS18800 (NFG60_18800) pilT 4428502..4429545 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  NFG60_RS18805 (NFG60_18805) - 4429573..4430259 (+) 687 WP_252118959.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFG60_RS18810 (NFG60_18810) proC 4430330..4431151 (+) 822 WP_110288223.1 pyrroline-5-carboxylate reductase -
  NFG60_RS18815 (NFG60_18815) - 4431172..4431708 (+) 537 WP_014950467.1 YggT family protein -
  NFG60_RS18820 (NFG60_18820) - 4431805..4432278 (+) 474 WP_252118960.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40839.73 Da        Isoelectric Point: 5.8993

>NTDB_id=697868 NFG60_RS18795 WP_252118958.1 4427381..4428493(-) (pilU) [Alteromonas macleodii strain QZ9-9]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEESLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=697868 NFG60_RS18795 WP_252118958.1 4427381..4428493(-) (pilU) [Alteromonas macleodii strain QZ9-9]
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCATCGGATTTATTCGTAACGGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAACTTACGCCAGTGACGGAAAGCGCGACCTCGGAAGAGGAATCGCTGGCGCTGGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAGGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACTCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAATCAACGTCATTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCACGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAGAACTGCGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCGACGACGC
CCTTAAAAGTTCGCTACGTCAGGCGCCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCTGATACAGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGCTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCTACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATACTGCTTAATTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCCAACGATTTGCGCTTGAT
GATCAAGCTTGATACCAGCGACGGTTCTAGCTTAGGTACACTATCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

56.793

99.459

0.565
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.892

0.392
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362