Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NAL94_RS10785 Genome accession   NZ_CP098034
Coordinates   587156..587587 (-) Length   143 a.a.
NCBI ID   WP_042521333.1    Uniprot ID   A0A7Y0MVX2
Organism   Vibrio alginolyticus strain E110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582156..592587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL94_RS10765 (NAL94_10765) coaE 582664..583278 (-) 615 WP_042521328.1 dephospho-CoA kinase -
  NAL94_RS10770 (NAL94_10770) pilD 583279..584148 (-) 870 WP_017635919.1 prepilin peptidase Machinery gene
  NAL94_RS10775 (NAL94_10775) pilC 584213..585436 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  NAL94_RS10780 (NAL94_10780) pilB 585460..587145 (-) 1686 WP_042521331.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NAL94_RS10785 (NAL94_10785) pilA 587156..587587 (-) 432 WP_042521333.1 pilin Machinery gene
  NAL94_RS10790 (NAL94_10790) nadC 587849..588736 (-) 888 WP_042521335.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NAL94_RS10795 (NAL94_10795) ampD 588829..589380 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NAL94_RS10800 (NAL94_10800) pdhR 589786..590553 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14577.45 Da        Isoelectric Point: 4.6894

>NTDB_id=693690 NAL94_RS10785 WP_042521333.1 587156..587587(-) (pilA) [Vibrio alginolyticus strain E110]
MKNSKQKKQQGFTLIELMIVVAIIGVLAAVAIPAYQDYVKKSEAASALATLKSLITPAELYYQENGTSSAAGLSDLGSVA
SANDLGTLTSAIGSGSGAVPTLEFAFGSNSSMTSSDTLTFSRSATEGWSCARAGTVPAIDGCQ

Nucleotide


Download         Length: 432 bp        

>NTDB_id=693690 NAL94_RS10785 WP_042521333.1 587156..587587(-) (pilA) [Vibrio alginolyticus strain E110]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATCGAATTGATGATTGTGGTGGCGATTATTGGTGTTTT
GGCAGCCGTCGCCATACCAGCTTATCAAGACTACGTAAAAAAATCAGAAGCCGCATCAGCTCTTGCTACGTTAAAGTCCC
TAATTACTCCGGCTGAACTCTATTATCAAGAAAATGGAACGAGCTCGGCTGCTGGTCTTTCTGATTTAGGTTCAGTAGCT
TCAGCTAACGATTTAGGCACACTAACATCAGCAATTGGATCTGGTTCAGGTGCGGTACCAACTCTAGAATTTGCTTTTGG
TTCAAATAGCTCAATGACCTCGAGTGATACTTTAACTTTCTCTCGTAGTGCAACCGAAGGTTGGTCTTGTGCTCGTGCTG
GTACAGTCCCAGCAATTGATGGCTGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0MVX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

62.676

99.301

0.622
  pilA Pseudomonas aeruginosa PAK

44.776

93.706

0.42
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.606

95.804

0.399
  pilA Vibrio cholerae strain A1552

41.606

95.804

0.399
  pilA Vibrio cholerae C6706

41.606

95.804

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

95.105

0.392