Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NAF16_RS01720 Genome accession   NZ_CP098021
Coordinates   359111..360799 (-) Length   562 a.a.
NCBI ID   WP_158129991.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354111..365799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS01695 (NAF16_01695) yacG 355292..355489 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  NAF16_RS01700 (NAF16_01700) zapD 355544..356284 (-) 741 WP_020332196.1 cell division protein ZapD -
  NAF16_RS01705 (NAF16_01705) coaE 356317..356925 (-) 609 WP_158129989.1 dephospho-CoA kinase -
  NAF16_RS01710 (NAF16_01710) pilD 356928..357797 (-) 870 WP_047459100.1 A24 family peptidase Machinery gene
  NAF16_RS01715 (NAF16_01715) pilC 357849..359075 (-) 1227 WP_158125405.1 type II secretion system F family protein Machinery gene
  NAF16_RS01720 (NAF16_01720) pilB 359111..360799 (-) 1689 WP_158129991.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NAF16_RS01725 (NAF16_01725) - 360813..361229 (-) 417 WP_158129992.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NAF16_RS01730 (NAF16_01730) nadC 361461..362348 (-) 888 WP_055452471.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NAF16_RS01735 (NAF16_01735) ampD 362501..363052 (+) 552 WP_323694950.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NAF16_RS01740 (NAF16_01740) fldB 363057..363578 (-) 522 WP_020430452.1 flavodoxin FldB -
  NAF16_RS01745 (NAF16_01745) xerD 363719..364627 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  NAF16_RS01750 (NAF16_01750) dsbC 364644..365408 (+) 765 WP_323694951.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61997.85 Da        Isoelectric Point: 6.2642

>NTDB_id=693542 NAF16_RS01720 WP_158129991.1 359111..360799(-) (pilB) [Vibrio fluvialis strain V13]
MLTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFHHHDLTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAITSHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=693542 NAF16_RS01720 WP_158129991.1 359111..360799(-) (pilB) [Vibrio fluvialis strain V13]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGAACCAATGTGCCGGAGGCGTTGATCGATTTGGCGATATTCCATCACCACGATCTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCGCTTTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGATACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAGTAGAACTGGTATTAGCTGATCATAAAGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCACGTCACATGGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAAATCCTGCTTGACGCGGTGCGTAAAGGCGCTTCTGATATCCATTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGCATTTTGGTGGAAACCCAGCAACCAGCCAGCCATTTAAGCCGCCGACTGGCTGCGCGGATTAAGATC
TTATCGAAACTCGATATCGCTGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGCGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCGTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCATTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.918

100

0.829
  pilB Vibrio campbellii strain DS40M4

72.42

100

0.724
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.611

91.993

0.484
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilB Acinetobacter baylyi ADP1

48.387

93.772

0.454
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.544

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368