Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9410_RS02505 Genome accession   NZ_CP097799
Coordinates   535072..536457 (+) Length   461 a.a.
NCBI ID   WP_005755879.1    Uniprot ID   -
Organism   Pasteurella multocida strain 39639     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530072..541457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9410_RS02475 (M9410_02475) - 530263..531114 (-) 852 WP_005751072.1 thymidylate synthase -
  M9410_RS02480 (M9410_02480) lgt 531120..531923 (-) 804 WP_005755876.1 prolipoprotein diacylglyceryl transferase -
  M9410_RS02485 (M9410_02485) - 531933..532727 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9410_RS02490 (M9410_02490) rppH 532730..533326 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9410_RS02495 (M9410_02495) ampD 533912..534478 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9410_RS02500 (M9410_02500) pilA 534638..535072 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9410_RS02505 (M9410_02505) pilB 535072..536457 (+) 1386 WP_005755879.1 GspE/PulE family protein Machinery gene
  M9410_RS02510 (M9410_02510) pilC 536457..537668 (+) 1212 WP_005755881.1 type II secretion system F family protein Machinery gene
  M9410_RS02515 (M9410_02515) - 537668..538390 (+) 723 WP_005755882.1 A24 family peptidase -
  M9410_RS02520 (M9410_02520) coaE 538405..539025 (+) 621 WP_005751078.1 dephospho-CoA kinase -
  M9410_RS02525 (M9410_02525) yacG 539036..539239 (+) 204 WP_005755884.1 DNA gyrase inhibitor YacG -
  M9410_RS02530 (M9410_02530) - 539239..539508 (+) 270 WP_005755886.1 GNAT family N-acetyltransferase -
  M9410_RS02535 (M9410_02535) - 539957..541330 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52155.83 Da        Isoelectric Point: 6.5002

>NTDB_id=692683 M9410_RS02505 WP_005755879.1 535072..536457(+) (pilB) [Pasteurella multocida strain 39639]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692683 M9410_RS02505 WP_005755879.1 535072..536457(+) (pilB) [Pasteurella multocida strain 39639]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACCGGGATAATCCAGACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGCGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

40

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

39.752

100

0.416
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.412
  pilB Vibrio cholerae strain A1552

38.809

100

0.41
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401