Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9414_RS02615 Genome accession   NZ_CP097795
Coordinates   556677..558065 (+) Length   462 a.a.
NCBI ID   WP_250269424.1    Uniprot ID   -
Organism   Pasteurella multocida strain 34030     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551677..563065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9414_RS02585 (M9414_02585) - 551868..552719 (-) 852 WP_115097855.1 thymidylate synthase -
  M9414_RS02590 (M9414_02590) lgt 552725..553528 (-) 804 WP_115097854.1 prolipoprotein diacylglyceryl transferase -
  M9414_RS02595 (M9414_02595) - 553538..554332 (-) 795 WP_115097853.1 sulfite exporter TauE/SafE family protein -
  M9414_RS02600 (M9414_02600) rppH 554335..554931 (-) 597 WP_115097852.1 RNA pyrophosphohydrolase -
  M9414_RS02605 (M9414_02605) ampD 555517..556083 (-) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9414_RS02610 (M9414_02610) pilA 556243..556677 (+) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  M9414_RS02615 (M9414_02615) pilB 556677..558065 (+) 1389 WP_250269424.1 GspE/PulE family protein Machinery gene
  M9414_RS02620 (M9414_02620) pilC 558065..559276 (+) 1212 WP_250269514.1 type II secretion system F family protein Machinery gene
  M9414_RS02625 (M9414_02625) - 559276..560001 (+) 726 WP_250269425.1 A24 family peptidase -
  M9414_RS02630 (M9414_02630) coaE 560013..560633 (+) 621 WP_104227613.1 dephospho-CoA kinase -
  M9414_RS02635 (M9414_02635) yacG 560644..560847 (+) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  M9414_RS02640 (M9414_02640) - 560847..561116 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9414_RS02645 (M9414_02645) - 561565..562938 (+) 1374 WP_096743226.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52060.71 Da        Isoelectric Point: 6.6308

>NTDB_id=692595 M9414_RS02615 WP_250269424.1 556677..558065(+) (pilB) [Pasteurella multocida strain 34030]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
KNELKRALQQLSPPKLQVEESIPTAYQVVQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRLQHVLSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVSLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=692595 M9414_RS02615 WP_250269424.1 556677..558065(+) (pilB) [Pasteurella multocida strain 34030]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAGCCTGTTTTATTATCA
AAAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTAGAAGAAAGCATACCCACGGCTTATCA
AGTCGTGCAATTAGATTCTGATTCCGCAACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGCAGACCAACAACACAGGCTCCAACACGTGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTAGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.052

0.667
  pilB Haemophilus influenzae Rd KW20

67.55

98.052

0.662
  pilB Glaesserella parasuis strain SC1401

58.134

99.784

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.42
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.413
  pilB Acinetobacter baumannii D1279779

38.571

100

0.409
  pilB Vibrio cholerae strain A1552

38.683

100

0.407
  pilB Acinetobacter baylyi ADP1

37.275

100

0.403
  pilB Legionella pneumophila strain ERS1305867

36.196

100

0.383