Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9414_RS02610 Genome accession   NZ_CP097795
Coordinates   556243..556677 (+) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain 34030     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551243..561677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9414_RS02580 (M9414_02580) tadA 551353..551841 (-) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -
  M9414_RS02585 (M9414_02585) - 551868..552719 (-) 852 WP_115097855.1 thymidylate synthase -
  M9414_RS02590 (M9414_02590) lgt 552725..553528 (-) 804 WP_115097854.1 prolipoprotein diacylglyceryl transferase -
  M9414_RS02595 (M9414_02595) - 553538..554332 (-) 795 WP_115097853.1 sulfite exporter TauE/SafE family protein -
  M9414_RS02600 (M9414_02600) rppH 554335..554931 (-) 597 WP_115097852.1 RNA pyrophosphohydrolase -
  M9414_RS02605 (M9414_02605) ampD 555517..556083 (-) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9414_RS02610 (M9414_02610) pilA 556243..556677 (+) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  M9414_RS02615 (M9414_02615) pilB 556677..558065 (+) 1389 WP_250269424.1 GspE/PulE family protein Machinery gene
  M9414_RS02620 (M9414_02620) pilC 558065..559276 (+) 1212 WP_250269514.1 type II secretion system F family protein Machinery gene
  M9414_RS02625 (M9414_02625) - 559276..560001 (+) 726 WP_250269425.1 A24 family peptidase -
  M9414_RS02630 (M9414_02630) coaE 560013..560633 (+) 621 WP_104227613.1 dephospho-CoA kinase -
  M9414_RS02635 (M9414_02635) yacG 560644..560847 (+) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  M9414_RS02640 (M9414_02640) - 560847..561116 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=692594 M9414_RS02610 WP_083004804.1 556243..556677(+) (pilA) [Pasteurella multocida strain 34030]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=692594 M9414_RS02610 WP_083004804.1 556243..556677(+) (pilA) [Pasteurella multocida strain 34030]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361