NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M9413_RS02870	Genome accession	NZ_CP097794
Coordinates	609699..610133 (+)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain 28606
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 604699..615133

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M9413_RS02840 (M9413_02840)	tadA	604809..605309 (-)	501	WP_005722410.1	tRNA adenosine(34) deaminase TadA	-
M9413_RS02845 (M9413_02845)	-	605324..606175 (-)	852	WP_250265505.1	thymidylate synthase	-
M9413_RS02850 (M9413_02850)	lgt	606181..606984 (-)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
M9413_RS02855 (M9413_02855)	-	606994..607788 (-)	795	WP_250265506.1	sulfite exporter TauE/SafE family protein	-
M9413_RS02860 (M9413_02860)	rppH	607791..608387 (-)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
M9413_RS02865 (M9413_02865)	ampD	608973..609539 (-)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
M9413_RS02870 (M9413_02870)	pilA	609699..610133 (+)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
M9413_RS02875 (M9413_02875)	pilB	610133..611518 (+)	1386	WP_250265507.1	GspE/PulE family protein	Machinery gene
M9413_RS02880 (M9413_02880)	-	611518..612729 (+)	1212	WP_250265592.1	type II secretion system F family protein	-
M9413_RS02885 (M9413_02885)	-	612729..613451 (+)	723	WP_250265508.1	A24 family peptidase	-
M9413_RS02890 (M9413_02890)	coaE	613466..614086 (+)	621	WP_163884064.1	dephospho-CoA kinase	-
M9413_RS02895 (M9413_02895)	yacG	614097..614300 (+)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
M9413_RS02900 (M9413_02900)	-	614300..614569 (+)	270	WP_005722568.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=692575 M9413_RS02870 WP_005741096.1 609699..610133(+) (pilA) [Pasteurella multocida strain 28606]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=692575 M9413_RS02870 WP_005741096.1 609699..610133(+) (pilA) [Pasteurella multocida strain 28606]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0692575