Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9413_RS02875 Genome accession   NZ_CP097794
Coordinates   610133..611518 (+) Length   461 a.a.
NCBI ID   WP_250265507.1    Uniprot ID   -
Organism   Pasteurella multocida strain 28606     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 605133..616518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9413_RS02845 (M9413_02845) - 605324..606175 (-) 852 WP_250265505.1 thymidylate synthase -
  M9413_RS02850 (M9413_02850) lgt 606181..606984 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M9413_RS02855 (M9413_02855) - 606994..607788 (-) 795 WP_250265506.1 sulfite exporter TauE/SafE family protein -
  M9413_RS02860 (M9413_02860) rppH 607791..608387 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9413_RS02865 (M9413_02865) ampD 608973..609539 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9413_RS02870 (M9413_02870) pilA 609699..610133 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9413_RS02875 (M9413_02875) pilB 610133..611518 (+) 1386 WP_250265507.1 GspE/PulE family protein Machinery gene
  M9413_RS02880 (M9413_02880) - 611518..612729 (+) 1212 WP_250265592.1 type II secretion system F family protein -
  M9413_RS02885 (M9413_02885) - 612729..613451 (+) 723 WP_250265508.1 A24 family peptidase -
  M9413_RS02890 (M9413_02890) coaE 613466..614086 (+) 621 WP_163884064.1 dephospho-CoA kinase -
  M9413_RS02895 (M9413_02895) yacG 614097..614300 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9413_RS02900 (M9413_02900) - 614300..614569 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9413_RS02905 (M9413_02905) - 615018..616391 (+) 1374 WP_250265509.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52063.76 Da        Isoelectric Point: 6.2343

>NTDB_id=692576 M9413_RS02875 WP_250265507.1 610133..611518(+) (pilB) [Pasteurella multocida strain 28606]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPMVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692576 M9413_RS02875 WP_250265507.1 610133..611518(+) (pilB) [Pasteurella multocida strain 28606]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCATGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.15

98.482

0.573
  pilB Acinetobacter baumannii D1279779

40.53

100

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.419
  pilB Vibrio cholerae strain A1552

39.22

100

0.414
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.412
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401