Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9419_RS02455 Genome accession   NZ_CP097791
Coordinates   541328..542713 (+) Length   461 a.a.
NCBI ID   WP_064964977.1    Uniprot ID   -
Organism   Pasteurella multocida strain 41060     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536328..547713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9419_RS02425 (M9419_02425) - 536519..537370 (-) 852 WP_005751072.1 thymidylate synthase -
  M9419_RS02430 (M9419_02430) lgt 537376..538179 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M9419_RS02435 (M9419_02435) - 538189..538983 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9419_RS02440 (M9419_02440) rppH 538986..539582 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9419_RS02445 (M9419_02445) ampD 540168..540734 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9419_RS02450 (M9419_02450) pilA 540894..541328 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9419_RS02455 (M9419_02455) pilB 541328..542713 (+) 1386 WP_064964977.1 GspE/PulE family protein Machinery gene
  M9419_RS02460 (M9419_02460) - 542713..543924 (+) 1212 WP_059245778.1 type II secretion system F family protein -
  M9419_RS02465 (M9419_02465) - 543924..544646 (+) 723 WP_059245779.1 A24 family peptidase -
  M9419_RS02470 (M9419_02470) coaE 544661..545281 (+) 621 WP_059245780.1 dephospho-CoA kinase -
  M9419_RS02475 (M9419_02475) yacG 545292..545495 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9419_RS02480 (M9419_02480) - 545495..545764 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9419_RS02485 (M9419_02485) - 546213..547586 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52129.82 Da        Isoelectric Point: 6.2343

>NTDB_id=692509 M9419_RS02455 WP_064964977.1 541328..542713(+) (pilB) [Pasteurella multocida strain 41060]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLYPPKLQVEENIPMVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692509 M9419_RS02455 WP_064964977.1 541328..542713(+) (pilB) [Pasteurella multocida strain 41060]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTACCCACCAAAATTACAAGTGGAAGAAAACATACCCATGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.15

98.482

0.573
  pilB Acinetobacter baumannii D1279779

39.877

100

0.423
  pilB Acinetobacter baylyi ADP1

38.277

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.414
  pilB Vibrio cholerae strain A1552

38.889

100

0.41
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395
  pilF Thermus thermophilus HB27

38.426

93.709

0.36