Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9419_RS02450 Genome accession   NZ_CP097791
Coordinates   540894..541328 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 41060     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 535894..546328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9419_RS02420 (M9419_02420) tadA 536004..536504 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  M9419_RS02425 (M9419_02425) - 536519..537370 (-) 852 WP_005751072.1 thymidylate synthase -
  M9419_RS02430 (M9419_02430) lgt 537376..538179 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M9419_RS02435 (M9419_02435) - 538189..538983 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9419_RS02440 (M9419_02440) rppH 538986..539582 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9419_RS02445 (M9419_02445) ampD 540168..540734 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9419_RS02450 (M9419_02450) pilA 540894..541328 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9419_RS02455 (M9419_02455) pilB 541328..542713 (+) 1386 WP_064964977.1 GspE/PulE family protein Machinery gene
  M9419_RS02460 (M9419_02460) - 542713..543924 (+) 1212 WP_059245778.1 type II secretion system F family protein -
  M9419_RS02465 (M9419_02465) - 543924..544646 (+) 723 WP_059245779.1 A24 family peptidase -
  M9419_RS02470 (M9419_02470) coaE 544661..545281 (+) 621 WP_059245780.1 dephospho-CoA kinase -
  M9419_RS02475 (M9419_02475) yacG 545292..545495 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9419_RS02480 (M9419_02480) - 545495..545764 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=692508 M9419_RS02450 WP_005741096.1 540894..541328(+) (pilA) [Pasteurella multocida strain 41060]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=692508 M9419_RS02450 WP_005741096.1 540894..541328(+) (pilA) [Pasteurella multocida strain 41060]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368