NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M8856_RS05950	Genome accession	NZ_CP097622
Coordinates	1286289..1286723 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain P2723
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1281289..1291723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8856_RS05920 (M8856_05920)	-	1281854..1282123 (-)	270	WP_005755886.1	GNAT family N-acetyltransferase	-
M8856_RS05925 (M8856_05925)	yacG	1282123..1282325 (-)	203	Protein_1133	DNA gyrase inhibitor YacG	-
M8856_RS05930 (M8856_05930)	coaE	1282336..1282956 (-)	621	WP_005753357.1	dephospho-CoA kinase	-
M8856_RS05935 (M8856_05935)	-	1282971..1283693 (-)	723	WP_046333440.1	A24 family peptidase	-
M8856_RS05940 (M8856_05940)	-	1283693..1284904 (-)	1212	WP_250031998.1	type II secretion system F family protein	-
M8856_RS05945 (M8856_05945)	pilB	1284904..1286289 (-)	1386	WP_250031941.1	GspE/PulE family protein	Machinery gene
M8856_RS05950 (M8856_05950)	pilA	1286289..1286723 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
M8856_RS05955 (M8856_05955)	ampD	1286883..1287449 (+)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
M8856_RS05960 (M8856_05960)	rppH	1288035..1288631 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
M8856_RS05965 (M8856_05965)	-	1288634..1289428 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
M8856_RS05970 (M8856_05970)	lgt	1289438..1290250 (+)	813	WP_156732338.1	prolipoprotein diacylglyceryl transferase	-
M8856_RS05975 (M8856_05975)	-	1290247..1291097 (+)	851	Protein_1143	thymidylate synthase	-
M8856_RS05980 (M8856_05980)	tadA	1291112..1291612 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=691320 M8856_RS05950 WP_005741096.1 1286289..1286723(-) (pilA) [Pasteurella multocida strain P2723]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=691320 M8856_RS05950 WP_005741096.1 1286289..1286723(-) (pilA) [Pasteurella multocida strain P2723]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC

CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0691320