Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8856_RS05945 Genome accession   NZ_CP097622
Coordinates   1284904..1286289 (-) Length   461 a.a.
NCBI ID   WP_250031941.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2723     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1279904..1291289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8856_RS05915 (M8856_05915) - 1280033..1281405 (-) 1373 Protein_1131 sodium:alanine symporter family protein -
  M8856_RS05920 (M8856_05920) - 1281854..1282123 (-) 270 WP_005755886.1 GNAT family N-acetyltransferase -
  M8856_RS05925 (M8856_05925) yacG 1282123..1282325 (-) 203 Protein_1133 DNA gyrase inhibitor YacG -
  M8856_RS05930 (M8856_05930) coaE 1282336..1282956 (-) 621 WP_005753357.1 dephospho-CoA kinase -
  M8856_RS05935 (M8856_05935) - 1282971..1283693 (-) 723 WP_046333440.1 A24 family peptidase -
  M8856_RS05940 (M8856_05940) - 1283693..1284904 (-) 1212 WP_250031998.1 type II secretion system F family protein -
  M8856_RS05945 (M8856_05945) pilB 1284904..1286289 (-) 1386 WP_250031941.1 GspE/PulE family protein Machinery gene
  M8856_RS05950 (M8856_05950) pilA 1286289..1286723 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M8856_RS05955 (M8856_05955) ampD 1286883..1287449 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8856_RS05960 (M8856_05960) rppH 1288035..1288631 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M8856_RS05965 (M8856_05965) - 1288634..1289428 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M8856_RS05970 (M8856_05970) lgt 1289438..1290250 (+) 813 WP_156732338.1 prolipoprotein diacylglyceryl transferase -
  M8856_RS05975 (M8856_05975) - 1290247..1291097 (+) 851 Protein_1143 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52083.74 Da        Isoelectric Point: 6.3162

>NTDB_id=691319 M8856_RS05945 WP_250031941.1 1284904..1286289(-) (pilB) [Pasteurella multocida strain P2723]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCHV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=691319 M8856_RS05945 WP_250031941.1 1284904..1286289(-) (pilB) [Pasteurella multocida strain P2723]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGTTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCATTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCGCCCTCTGCCCTTACTCGTTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTGGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCATGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

57.86

99.349

0.575
  pilB Acinetobacter baumannii D1279779

39.798

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.421
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.412
  pilB Vibrio cholerae strain A1552

38.809

100

0.41
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401