NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M8850_RS06480	Genome accession	NZ_CP097610
Coordinates	1353486..1353920 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain 32985
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1348486..1358920

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8850_RS06450 (M8850_06450)	-	1349050..1349319 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
M8850_RS06455 (M8850_06455)	yacG	1349319..1349522 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
M8850_RS06460 (M8850_06460)	coaE	1349533..1350153 (-)	621	WP_005753357.1	dephospho-CoA kinase	-
M8850_RS06465 (M8850_06465)	-	1350168..1350890 (-)	723	WP_032851624.1	A24 family peptidase	-
M8850_RS06470 (M8850_06470)	-	1350890..1352101 (-)	1212	WP_005753354.1	type II secretion system F family protein	-
M8850_RS06475 (M8850_06475)	pilB	1352101..1353486 (-)	1386	WP_005753352.1	GspE/PulE family protein	Machinery gene
M8850_RS06480 (M8850_06480)	pilA	1353486..1353920 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
M8850_RS06485 (M8850_06485)	ampD	1354080..1354646 (+)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
M8850_RS06490 (M8850_06490)	rppH	1355232..1355828 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
M8850_RS06495 (M8850_06495)	-	1355831..1356625 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
M8850_RS06500 (M8850_06500)	lgt	1356635..1357438 (+)	804	WP_005753347.1	prolipoprotein diacylglyceryl transferase	-
M8850_RS06505 (M8850_06505)	-	1357444..1358295 (+)	852	WP_005753345.1	thymidylate synthase	-
M8850_RS06510 (M8850_06510)	tadA	1358310..1358810 (+)	501	WP_005753343.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=691194 M8850_RS06480 WP_005741096.1 1353486..1353920(-) (pilA) [Pasteurella multocida strain 32985]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=691194 M8850_RS06480 WP_005741096.1 1353486..1353920(-) (pilA) [Pasteurella multocida strain 32985]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0691194