Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8850_RS06475 Genome accession   NZ_CP097610
Coordinates   1352101..1353486 (-) Length   461 a.a.
NCBI ID   WP_005753352.1    Uniprot ID   -
Organism   Pasteurella multocida strain 32985     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1347101..1358486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8850_RS06445 (M8850_06445) - 1347228..1348601 (-) 1374 WP_005722571.1 sodium:alanine symporter family protein -
  M8850_RS06450 (M8850_06450) - 1349050..1349319 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M8850_RS06455 (M8850_06455) yacG 1349319..1349522 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M8850_RS06460 (M8850_06460) coaE 1349533..1350153 (-) 621 WP_005753357.1 dephospho-CoA kinase -
  M8850_RS06465 (M8850_06465) - 1350168..1350890 (-) 723 WP_032851624.1 A24 family peptidase -
  M8850_RS06470 (M8850_06470) - 1350890..1352101 (-) 1212 WP_005753354.1 type II secretion system F family protein -
  M8850_RS06475 (M8850_06475) pilB 1352101..1353486 (-) 1386 WP_005753352.1 GspE/PulE family protein Machinery gene
  M8850_RS06480 (M8850_06480) pilA 1353486..1353920 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M8850_RS06485 (M8850_06485) ampD 1354080..1354646 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8850_RS06490 (M8850_06490) rppH 1355232..1355828 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M8850_RS06495 (M8850_06495) - 1355831..1356625 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M8850_RS06500 (M8850_06500) lgt 1356635..1357438 (+) 804 WP_005753347.1 prolipoprotein diacylglyceryl transferase -
  M8850_RS06505 (M8850_06505) - 1357444..1358295 (+) 852 WP_005753345.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52023.63 Da        Isoelectric Point: 6.2343

>NTDB_id=691193 M8850_RS06475 WP_005753352.1 1352101..1353486(-) (pilB) [Pasteurella multocida strain 32985]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=691193 M8850_RS06475 WP_005753352.1 1352101..1353486(-) (pilB) [Pasteurella multocida strain 32985]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.37

98.482

0.575
  pilB Acinetobacter baumannii D1279779

39.959

100

0.427
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.683

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395