Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M5524_RS06870 Genome accession   NZ_CP097466
Coordinates   1583351..1585090 (-) Length   579 a.a.
NCBI ID   WP_373990706.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1578351..1590090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS06845 (M5524_06820) pilD 1578861..1579733 (-) 873 WP_373990701.1 A24 family peptidase Machinery gene
  M5524_RS06850 (M5524_06825) - 1579889..1580677 (+) 789 WP_373990702.1 sensor histidine kinase -
  M5524_RS06855 (M5524_06830) - 1580674..1581327 (-) 654 WP_373990703.1 response regulator -
  M5524_RS06860 (M5524_06835) - 1581490..1582122 (+) 633 WP_373990704.1 cysteine hydrolase family protein -
  M5524_RS06865 (M5524_06840) pilC 1582126..1583340 (-) 1215 WP_373990705.1 type II secretion system F family protein Machinery gene
  M5524_RS06870 (M5524_06845) pilF 1583351..1585090 (-) 1740 WP_373990706.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M5524_RS06875 (M5524_06850) - 1585221..1586501 (-) 1281 WP_373990707.1 HlyC/CorC family transporter -
  M5524_RS06880 (M5524_06855) - 1586884..1588377 (-) 1494 WP_373990708.1 tetratricopeptide repeat protein -
  M5524_RS06885 (M5524_06860) uraH 1588398..1588751 (-) 354 WP_373990709.1 hydroxyisourate hydrolase -
  M5524_RS06890 (M5524_06865) puuE 1588861..1589814 (+) 954 WP_373990710.1 allantoinase PuuE -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62249.06 Da        Isoelectric Point: 5.1903

>NTDB_id=689582 M5524_RS06870 WP_373990706.1 1583351..1585090(-) (pilF) [Duganella sp. BuS-21]
MAAVLPNAAPGAPMPGLARALLQAGRLSATQAESLQKKSVAEKLAFIDVLLSSGVIESKALAVFCAETFAYPLIDVSALS
IEALPAKIIDNKLMQAQRVIALAKRGNKMSVAISDPTNIQALDQIKFQTESTVEPVIVAHDALLQLLARLNKSSEQSIAD
LAGDEADIQFAEDEEPSAASAAAADAAANEVEDAPVVRFLNKMLIDAVNMGASDLHFEPFEKFYRIRFRVDGVLIEHAQP
PISIKEKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTIDLRISTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQK
ELLLDAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFPVALKSFLRQD
PDIIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCSCKQPVEIA
PELLIKAGYQPEELDGSWKPYGPVGCERCNGGGYKGRVGIYQIMPITPAIEALILAHGNAMDIAAKSEQEGVKSLRQSGL
VKVKAGMTSLEEVLGCTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=689582 M5524_RS06870 WP_373990706.1 1583351..1585090(-) (pilF) [Duganella sp. BuS-21]
ATGGCAGCAGTCCTTCCTAACGCAGCGCCAGGCGCCCCGATGCCGGGGTTGGCGAGGGCATTGCTGCAGGCCGGACGCCT
GTCCGCCACACAGGCCGAGAGCTTGCAAAAAAAATCCGTGGCCGAGAAGCTGGCCTTTATCGACGTGCTGCTGTCGAGCG
GCGTGATCGAATCGAAAGCCCTGGCCGTTTTCTGCGCCGAAACCTTCGCCTATCCCCTGATCGATGTCAGCGCGCTCAGC
ATTGAAGCGCTGCCGGCCAAAATCATCGACAACAAGCTGATGCAGGCCCAGCGCGTGATCGCCTTGGCCAAACGCGGCAA
CAAGATGTCGGTGGCGATTTCCGACCCCACCAATATCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGTCCACCGTCG
AACCGGTGATCGTGGCGCACGACGCGCTGCTGCAACTGCTGGCCCGCCTCAACAAGAGTAGCGAGCAGAGCATCGCCGAC
CTGGCCGGCGACGAGGCCGATATCCAGTTCGCCGAGGACGAGGAACCATCCGCCGCCAGCGCGGCCGCCGCCGATGCCGC
CGCCAACGAGGTCGAAGACGCGCCGGTGGTGCGCTTCCTCAACAAGATGCTGATCGACGCCGTCAATATGGGCGCCTCGG
ATTTGCATTTCGAACCGTTTGAAAAGTTTTACCGCATCCGCTTCCGCGTCGACGGCGTGCTGATCGAACATGCCCAGCCG
CCGATCTCGATCAAGGAAAAACTGGTGTCGCGCATCAAGGTGCTGGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCA
GGACGGCCGCATGCGCCTGATCGTCTCGCCGACCAAGACCATCGACCTGCGCATCTCCACCTTGCCGACCCTGTTCGGCG
AGAAAACCGTGATGCGGATCCTCGACGCCACCCAGGCGCAGATGGGCATCGACAGCCTCGGCTACGATCCCGACCAGAAG
GAGCTGCTGCTCGATGCCATCCAGCGTCCTTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCCGGCAAGACGGTGTC
GCTGTACACCTGCCTCAACATACTGAACAAGCCCGGCATCAACATCTCCACCGCCGAAGACCCGGCCGAGATCAACCTGC
CGGGCGTGAACCAGGTCAACGTCAACGACCGCGCCGGCCTGACCTTCCCGGTGGCGCTGAAGTCCTTCCTGCGCCAGGAC
CCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCGCAAACCGGCCACAT
GGTGTTCTCCACGCTGCACACCAACGACGCGCCGTCCACGCTGACGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACA
TCGCCTCGTCGGTGATTCTGATTACCGCCCAGCGCCTGGCGCGCCGCCTGTGCAGCTGCAAGCAGCCGGTCGAGATCGCC
CCCGAACTCTTGATCAAGGCCGGCTACCAGCCCGAGGAACTGGACGGCAGCTGGAAGCCCTACGGCCCGGTCGGCTGCGA
GCGCTGCAACGGCGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGATCATGCCGATCACGCCCGCCATCGAGGCGCTGA
TCCTGGCCCACGGCAACGCCATGGACATTGCCGCCAAGTCGGAACAAGAAGGCGTGAAGTCGCTGCGCCAGTCCGGCCTG
GTCAAGGTCAAGGCGGGCATGACCAGCCTGGAAGAAGTGCTCGGCTGTACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.169

98.1

0.522
  pilB Acinetobacter baylyi ADP1

52.297

97.755

0.511
  pilB Acinetobacter baumannii D1279779

52.12

97.755

0.509
  pilB Legionella pneumophila strain ERS1305867

46.737

97.927

0.458
  pilB Vibrio cholerae strain A1552

45.936

97.755

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.237

0.447
  pilB Vibrio campbellii strain DS40M4

44.346

97.755

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.051

94.646

0.37
  pilF Thermus thermophilus HB27

39.405

92.919

0.366