Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M4X66_RS21270 Genome accession   NZ_CP097286
Coordinates   4824690..4826789 (-) Length   699 a.a.
NCBI ID   WP_057410477.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain JACO-C-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4819690..4831789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4X66_RS21255 - 4821300..4822928 (-) 1629 WP_249582961.1 AAA family ATPase -
  M4X66_RS21260 aroB 4822938..4824041 (-) 1104 WP_122552508.1 3-dehydroquinate synthase -
  M4X66_RS21265 aroK 4824167..4824685 (-) 519 WP_004882714.1 shikimate kinase AroK -
  M4X66_RS21270 pilQ 4824690..4826789 (-) 2100 WP_057410477.1 type IV pilus secretin PilQ Machinery gene
  M4X66_RS21275 - 4826837..4827364 (-) 528 WP_004882716.1 pilus assembly protein PilP -
  M4X66_RS21280 pilO 4827361..4827984 (-) 624 WP_004882717.1 type 4a pilus biogenesis protein PilO -
  M4X66_RS21285 - 4827981..4828559 (-) 579 WP_004882718.1 PilN domain-containing protein -
  M4X66_RS21290 pilM 4828559..4829623 (-) 1065 WP_004882719.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75358.75 Da        Isoelectric Point: 4.9910

>NTDB_id=688270 M4X66_RS21270 WP_057410477.1 4824690..4826789(-) (pilQ) [Pseudomonas viridiflava strain JACO-C-5]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAKGAARVAVPPPTPASARPYVPA
GVRAIRNVDFQRGELGEGNVVIDLSNASIAADIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SVGGLDDNGDEAGNTGNDLTANIPFVDLGAPDATAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVAEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=688270 M4X66_RS21270 WP_057410477.1 4824690..4826789(-) (pilQ) [Pseudomonas viridiflava strain JACO-C-5]
ATGACCCGGATTCTCTCGATTATCGGCGTTTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCCGCCAATCTCAA
GACGCTCGACGTCGCGGCCCTGCCGGGTGATCGAATCGAGCTCAAGCTTTCCTTCGACGCACCGGTGCCCGCGCCACGCG
GCTATACCACCGAGCAGCCTGCGCGCATTGCGCTGGACCTGCCGGGCGTGACCAGCCAGCTGGCGACCAAGAGTCGTGAT
CTGGGGGCAGGCAATGCCCGCAGCGTGGTGGTTGTGCAGGCGCAGGACCGTACGCGGGTCATCATCAACCTGACGACGCT
TTCGCCGTACAGCTCACGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGTCAGGGGGCTGCAGCAGCTCAGGCTTCAC
AGCCGACCACCGCCAAGGGCGCTGCACGGGTAGCCGTGCCGCCGCCAACACCGGCATCGGCGCGGCCCTACGTGCCTGCG
GGCGTCAGAGCCATTCGCAATGTCGACTTCCAGCGCGGCGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAGCATCGCTGCAGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGATTTCGCCAAGACGCAATTGCCCGATCCGCTGC
GCGTGCGTCTGGACGTGAAAGACTTCGCCACACCCGTGCAATTCGTCAGCGCCACGGCGTCCGGCGACAAGGCCAGCATT
TCCATCGAGCCATCGGGTGCGTTTGATTATTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTGCGCCCATTGAC
CAATGAGGACGTGGATCGTCGCGCCGCCGACAAGCCGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTTCAGGACATCG
ATGTGCGTTCGGTACTGCAACTGATCGCCGATTTCACCAACCTCAATCTGGTTGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGT
CGGCAGCGTGCTGCTGGTCGCCCCGGCTGACGAGATCGCCGCGCGTGAGCGTCAGGAACTGGAGTCGCTCAAGCAGATTG
CCGAACTGGCCCCGTTGCGTCGTGAGCTGTTGCAGGTCAACTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCG
GTCACCAGCGCCGAGTCCAAGGCGGACGAGCGTGGTTCCATTACTGTGGATGAACGCACCAACAACATCATTGCGTATCA
GACTCAGGATCGACTCGACGAGCTGCGCCGTATCGTCTCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGC
GGATCGTTGAAGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGGCGAACCGACCGGTCGCGCAAGTGG
AGTGTCGGCGGCCTTGACGATAACGGCGATGAAGCAGGCAATACGGGCAACGATCTGACGGCCAACATTCCGTTTGTGGA
TCTGGGTGCTCCAGATGCCACGGCGGGTGTCGGGATCGGTTTCCTGACCAACAATGCACTGCTGGACCTTGAGCTGAGCG
CGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACTGCCAAGATCCTC
AAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACGACAGTGAGCTTCAAGGAGGCGTCCCTGTCGCT
TGAGGTCACGCCACAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCTGATTACCTCA
ATGCGGTGCTGGGCGTGCCTCCTATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACCATCGTG
ATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTGGCAGAGGCGAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.399

100

0.747
  comQ Acinetobacter baylyi ADP1

40.656

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.756

100

0.395
  pilQ Acinetobacter baumannii strain A118

37.756

100

0.395
  pilQ Legionella pneumophila strain Lp02

38.067

97.711

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.067

97.711

0.372