Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   M4X66_RS21290 Genome accession   NZ_CP097286
Coordinates   4828559..4829623 (-) Length   354 a.a.
NCBI ID   WP_004882719.1    Uniprot ID   A0A0D0MR84
Organism   Pseudomonas viridiflava strain JACO-C-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4823559..4834623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4X66_RS21265 aroK 4824167..4824685 (-) 519 WP_004882714.1 shikimate kinase AroK -
  M4X66_RS21270 pilQ 4824690..4826789 (-) 2100 WP_057410477.1 type IV pilus secretin PilQ Machinery gene
  M4X66_RS21275 - 4826837..4827364 (-) 528 WP_004882716.1 pilus assembly protein PilP -
  M4X66_RS21280 pilO 4827361..4827984 (-) 624 WP_004882717.1 type 4a pilus biogenesis protein PilO -
  M4X66_RS21285 - 4827981..4828559 (-) 579 WP_004882718.1 PilN domain-containing protein -
  M4X66_RS21290 pilM 4828559..4829623 (-) 1065 WP_004882719.1 pilus assembly protein PilM Machinery gene
  M4X66_RS21295 - 4829830..4832274 (+) 2445 WP_249582962.1 penicillin-binding protein 1A -
  M4X66_RS21300 - 4832671..4833126 (+) 456 WP_029243170.1 YkgJ family cysteine cluster protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38031.45 Da        Isoelectric Point: 4.7090

>NTDB_id=688271 M4X66_RS21290 WP_004882719.1 4828559..4829623(-) (pilM) [Pseudomonas viridiflava strain JACO-C-5]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVVGKAKTSARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSLRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=688271 M4X66_RS21290 WP_004882719.1 4828559..4829623(-) (pilM) [Pseudomonas viridiflava strain JACO-C-5]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGTTCCACCTCGGTCAAGCTACTTGAATT
GAGTCGTTCCGGAACCCGCTACAAAGTCGAGTCTTACGCTGTTGAGCCGTTGCCGGCCAATGCCGTCGTCGAGAAGAACA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCACGGGTCGTTGGCAAGGCCAAGACCAGCGCCAGAATAGTGGCGGTT
GCTGTCGCAGGCTCCGCAGTCATCACCAAGACGATCGAAATGGATGCCGGTCTCTCGGACGACGACATGGAAAATCAGCT
CAAGCTCGAGGCTGACCAGTACATCCCCTATCCCCTTGAAGAAGTGGCCATCGATTTCGAAGTGCAGGGCTATTCGCTGC
GCAACCCCGAACGCGTGGAAGTCCTTCTGGCTGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCCGCGCTGGCGCTG
GCTGGCCTCACCGCAAAAGTCGTTGATGTCGAAGCCTATGCCCTTGAGCGTTCGTTCGGGCTGCTGGCTGCGCAACTGGG
CAATGGTCACGATGAGCTGACCGTTGCGGTCGTCGATATCGGCGCGACCATGACCACACTGAGCGTTCTGCACCACGGCC
GGATCATCTACACCCGCGAGCAACTGTTCGGCGGGCGCCAGCTGACCGAAGAAATCCAGCGTCGCTACGGCCTGTCGATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCGGACGACTATGTCAGTGAAGTGCTGGAACCCTTCAAGGATGC
GCTGGTGCAGCAGGTCTCGCGCTCGCTTCAATTCTTCTTCGCGGCAGGCCAGTACAACTCTGTGGATCACATCATGCTCG
CTGGCGGTACGGCTTCCATTTCCGGCCTGGAGCATCTGATTCAGCGCCGACTCGGCACGCCGACCCAGGTGGCCAACCCG
TTTGCCGACATGGCCCTCAGCTCCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATCGCGTGCGG
TCTTGCTTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D0MR84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.887

100

0.48