Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M2I89_RS25520 Genome accession   NZ_CP096937
Coordinates   5384004..5385704 (+) Length   566 a.a.
NCBI ID   WP_003112841.1    Uniprot ID   P22608
Organism   Pseudomonas aeruginosa strain NY5520     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5365254..5383043 5384004..5385704 flank 961


Gene organization within MGE regions


Location: 5365254..5385704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I89_RS25410 (M2I89_25350) - 5365254..5365796 (-) 543 WP_123809552.1 hypothetical protein -
  M2I89_RS25415 (M2I89_25355) - 5365940..5366140 (+) 201 WP_023082007.1 helix-turn-helix domain-containing protein -
  M2I89_RS25420 (M2I89_25360) - 5366137..5366553 (+) 417 WP_034054964.1 hypothetical protein -
  M2I89_RS25425 (M2I89_25365) - 5366546..5366779 (+) 234 WP_031653969.1 hypothetical protein -
  M2I89_RS25430 (M2I89_25370) - 5366776..5367024 (+) 249 WP_031653970.1 hypothetical protein -
  M2I89_RS25435 (M2I89_25375) - 5367021..5367326 (+) 306 WP_023109345.1 hypothetical protein -
  M2I89_RS31925 - 5367329..5367766 (+) 438 WP_308416434.1 hypothetical protein -
  M2I89_RS25445 (M2I89_25385) - 5367805..5368272 (+) 468 WP_031629313.1 hypothetical protein -
  M2I89_RS25450 (M2I89_25390) - 5368279..5369232 (+) 954 WP_308416433.1 DUF3631 domain-containing protein -
  M2I89_RS25455 (M2I89_25395) - 5369232..5369525 (+) 294 WP_221345446.1 hypothetical protein -
  M2I89_RS25460 (M2I89_25400) - 5369581..5370477 (+) 897 WP_034069047.1 hypothetical protein -
  M2I89_RS25465 (M2I89_25405) - 5371183..5372358 (+) 1176 WP_223822176.1 DUF262 domain-containing protein -
  M2I89_RS25470 (M2I89_25410) - 5372369..5373196 (+) 828 WP_124157035.1 MAE_28990/MAE_18760 family HEPN-like nuclease -
  M2I89_RS25475 (M2I89_25415) - 5374510..5375016 (-) 507 WP_034064628.1 GNAT family N-acetyltransferase -
  M2I89_RS25480 (M2I89_25420) - 5375789..5376058 (+) 270 WP_225007575.1 hypothetical protein -
  M2I89_RS25485 (M2I89_25425) - 5376058..5376294 (+) 237 WP_003096116.1 hypothetical protein -
  M2I89_RS25490 (M2I89_25430) - 5376433..5376669 (-) 237 WP_071536299.1 hypothetical protein -
  M2I89_RS25495 (M2I89_25435) - 5376697..5379459 (-) 2763 WP_057379627.1 RHS repeat domain-containing protein -
  M2I89_RS25500 (M2I89_25440) - 5379474..5380622 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  M2I89_RS25505 (M2I89_25445) - 5382801..5383043 (+) 243 WP_023109353.1 hypothetical protein -
  M2I89_RS25515 (M2I89_25455) pilA 5383320..5383772 (-) 453 WP_028681203.1 pilin Machinery gene
  M2I89_RS25520 (M2I89_25460) pilB 5384004..5385704 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62328.19 Da        Isoelectric Point: 5.7041

>NTDB_id=685400 M2I89_RS25520 WP_003112841.1 5384004..5385704(+) (pilB) [Pseudomonas aeruginosa strain NY5520]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQSKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=685400 M2I89_RS25520 WP_003112841.1 5384004..5385704(+) (pilB) [Pseudomonas aeruginosa strain NY5520]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACTTGGTGCAGAGCAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTTGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTCTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGGAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22608

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.75

99.117

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366